植物促生根际细菌在盐碱条件下提高紫花苜蓿产量和品质。

Enhancement of alfalfa yield and quality by plant growth-promoting rhizobacteria under saline-alkali conditions.

机构信息

State Key Laboratory of Molecular Genetics, Academy of Life Science and Technology, Harbin Normal University, Harbin, China.

出版信息

J Sci Food Agric. 2019 Jan 15;99(1):281-289. doi: 10.1002/jsfa.9185. Epub 2018 Aug 22.

DOI:10.1002/jsfa.9185

PMID:29855046

Abstract

BACKGROUND

Bacteria with 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity could decrease the ethylene level, confer resistance of plant, and stimulate plant growth under biotic and abiotic stress conditions.

RESULTS

Plant growth-promoting rhizobacteria (PGPR) strains Enterobacter aerogenes (LJL-5) and Pseudomonas aeruginosa (LJL-13) were obtained from the rhizosphere of alfalfa grown under saline-alkali conditions. The ACC deaminase activity of E. aerogenes (LJL-5) and Ps. aeruginosa (LJL-13) was approximately 2-5 µmol mg h . indole acetic acid synthesis was increased with the increasing concentration of l-tryptophan. Siderophore production and phosphate solubilization in Ps. aeruginosa (LJL-13) were higher than those in E. aerogenes (LJL-5). Compared to the non-inoculated seedlings (1.31 ng mL h ), inoculated alfalfa seedlings with E. aerogenes (LJL-5) (0.90 ng mL h ) and Ps. aeruginosa (LJL-13) (0.78 ng mL h ) emitted lower levels of ethylene. Under saline-alkali conditions in the greenhouse, inoculation with E. aerogenes (LJL-5) and Ps. aeruginosa (LJL-13) increased the biomass, soil and plant analyzer development (SPAD), and P content of alfalfa plants, and also induced the activity of antioxidant enzymes (superoxide dismutase, peroxidase and catalase), promoted the accumulation of antioxidant substances and removed various harmful substances. Under saline-alkali conditions in the field (2012, 2013, and 2014), inoculation of alfalfa with E. aerogenes (LJL-5) and Ps. aeruginosa (LJL-13) significantly increased the shoot height, fresh and dry weights, yield and crude protein content of alfalfa plants, but decreased the fiber content.

CONCLUSION

Two PGPR strains were isolated from the rhizosphere of alfalfa in saline-alkali conditions. Both strains could promote alfalfa growth in saline-alkali soil, and could be used as biofertilizer to promote plant growth under stress and reduce environmental pollution caused by fertilizers simultaneously. © 2018 Society of Chemical Industry.

摘要

背景

具有 1-氨基环丙烷-1-羧酸（ACC）脱氨酶活性的细菌可以降低乙烯水平，赋予植物对生物和非生物胁迫条件的抗性，并刺激植物生长。

结果

从盐碱性条件下生长的紫花苜蓿根际中获得了植物促生根际细菌（PGPR）菌株肠杆菌（LJL-5）和铜绿假单胞菌（LJL-13）。肠杆菌（LJL-5）和铜绿假单胞菌（LJL-13）的 ACC 脱氨酶活性约为 2-5 μmol·mg -1 ·h -1 。吲哚乙酸的合成随着 l-色氨酸浓度的增加而增加。铜绿假单胞菌（LJL-13）的铁载体合成和磷酸盐溶解能力均高于肠杆菌（LJL-5）。与未接种的幼苗（1.31 ng·mL -1 ·h -1 ）相比，接种肠杆菌（LJL-5）（0.90 ng·mL -1 ·h -1 ）和铜绿假单胞菌（LJL-13）（0.78 ng·mL -1 ·h -1 ）的紫花苜蓿幼苗产生的乙烯水平较低。在温室的盐碱性条件下，接种肠杆菌（LJL-5）和铜绿假单胞菌（LJL-13）可增加紫花苜蓿植物的生物量、土壤和植物分析器发育（SPAD）和磷含量，并诱导抗氧化酶（超氧化物歧化酶、过氧化物酶和过氧化氢酶）的活性，促进抗氧化物质的积累，清除各种有害物质。在田间的盐碱性条件下（2012 年、2013 年和 2014 年），接种肠杆菌（LJL-5）和铜绿假单胞菌（LJL-13）可显著增加紫花苜蓿植株的茎高、鲜重和干重、产量和粗蛋白含量，但降低了纤维含量。