Center for Reproductive Medicine, Shandong University, Shandong, China.
Int J Gynaecol Obstet. 2018 Sep;142(3):354-358. doi: 10.1002/ijgo.12537. Epub 2018 Jun 25.
To evaluate the efficiency of spermatozoa cryopreservation and to compare the clinical pregnancy outcomes in intracytoplasmic sperm injection (ICSI) using fresh versus cryopreserved spermatozoa collected by testicular sperm aspiration (TESA).
A retrospective study was performed to compare the outcomes of men who accepted frozen-spermatozoa-based TESA-ICSI with those of men who underwent TESA-ICSI using fresh spermatozoa between January 1, 2015, and December 30, 2016. The groups were matched for age. The rates of fertilization, good-quality embryos, blastocyst formation, and clinical pregnancy outcomes were obtained from clinical records and were compared between the groups.
There were no significant differences between the frozen TESA group (n=79) and the fresh TESA group (n=194) in the rates of fertilization (71.4% vs 73.4%), good-quality embryos (55.3% vs 54.5%), blastocyst formation (60.9% vs 60.1%), clinical pregnancy (61.7% vs 55.1%), and live delivery (51.1% vs 45.7%) (P>0.05 for all comparisons).
Freezing low-count sperm collected by TESA with a cryoprotectant was an efficient method in the treatment of male factor infertility.
评估精子冷冻保存的效率，并比较经睾丸精子抽吸术（TESA）收集的新鲜精子和冷冻精子进行胞浆内单精子注射（ICSI）的临床妊娠结局。
本研究回顾性分析了 2015 年 1 月 1 日至 2016 年 12 月 30 日期间接受基于冷冻精子的 TESA-ICSI 和新鲜精子 TESA-ICSI 的男性患者的结局。两组患者按年龄匹配。从临床记录中获得受精率、优质胚胎率、囊胚形成率和临床妊娠结局，并进行组间比较。
冷冻 TESA 组（n=79）与新鲜 TESA 组（n=194）的受精率（71.4% vs 73.4%）、优质胚胎率（55.3% vs 54.5%）、囊胚形成率（60.9% vs 60.1%）、临床妊娠率（61.7% vs 55.1%）和活产率（51.1% vs 45.7%）差异均无统计学意义（P>0.05）。
用冷冻保护剂冷冻 TESA 收集的低计数精子是治疗男性因素不孕的有效方法。
