缝合切除术对家族性双冠状缝颅缝早闭兔脑血流与年龄相关变化的影响：定量分析。
The influence of suturectomy on age-related changes in cerebral blood flow in rabbits with familial bicoronal suture craniosynostosis: A quantitative analysis.
机构信息
Department of Neurosurgery, University of Texas Health Science Center at San Antonio, San Antonio, TX, United States of America.
Pittsburgh NMR Center for Biomedical Research, Carnegie Mellon University, Pittsburgh, PA, United States of America.
出版信息
PLoS One. 2018 Jun 1;13(6):e0197296. doi: 10.1371/journal.pone.0197296. eCollection 2018.
BACKGROUND
Coronal suture synostosis is a condition which can have deleterious physical and cognitive sequelae in humans if not corrected. A well-established animal model has previously demonstrated disruptions in intracranial pressure and developmental abnormalities in rabbits with congenital craniosynostosis compared to wild type rabbits.
OBJECTIVE
The current study aimed to measure the cerebral blood flow (CBF) in developing rabbits with craniosynostosis who underwent suturectomy compared to those with no intervention and compared to wild type rabbits.
METHODS
Rabbits with early onset coronal suture synostosis were assigned to have suturectomy at 10 days of age (EOCS-SU, n = 15) or no intervention (EOCS, n = 18). A subset of each group was randomly selected for measurement at 10 days of age, 25 days of age, and 42 days of age. Wild type rabbits (WT, n = 18) were also randomly assigned to measurement at each time point as controls. Cerebral blood flow at the bilateral hemispheres, cortices, thalami, and superficial cortices was measured in each group using arterial spin-labeling MRI.
RESULTS
At 25 days of age, CBF at the superficial cortex was significantly higher in EOCS rabbits (192.6 ± 10.1 mL/100 mg/min on the left and 195 ± 9.5 mL/100 mg/min on the right) compared to WT rabbits (99.2 ± 29.1 mL/100 mg/min on the left and 96.2 ± 21.4 mL/100 mg/min on the right), but there was no significant difference in CBF between EOCS-SU (97.6 ± 11.3 mL/100 mg/min on the left and 99 ± 7.4 mL/100 mg/min on the right) and WT rabbits. By 42 days of age the CBF in EOCS rabbits was not significantly different than that of WT rabbits.
CONCLUSION
Suturectomy eliminated the abnormally increased CBF at the superficial cortex seen in EOCS rabbits at 25 days of age. This finding contributes to the evidence that suturectomy limits abnormalities of ICP and CBF associated with craniosynostosis.
背景
冠状缝融合是一种病症，如果不加以纠正，会对人类的身体和认知产生有害影响。以前的一项成熟的动物模型研究表明，与野生型兔子相比，患有先天性颅缝早闭的兔子的颅内压会出现紊乱，并且在发育过程中会出现异常。
目的
本研究旨在测量患有冠状缝早闭的发育中兔子的脑血流（CBF），这些兔子接受了缝切除术（EOCS-SU，n=15），或未接受干预（EOCS，n=18），并与野生型兔子（WT，n=18）进行比较。每个组的一部分兔子会在 10 天、25 天和 42 天随机选择进行测量。
方法
将患有早期冠状缝融合的兔子分配在 10 天大时进行缝切除术（EOCS-SU，n=15）或不进行干预（EOCS，n=18）。每个组的一部分兔子会在 10 天、25 天和 42 天随机选择进行测量。每个组的双侧半球、皮质、丘脑和皮质表面的脑血流均使用动脉自旋标记 MRI 进行测量。
结果
在 25 天大时，EOCS 兔子的皮质表面脑血流（左侧 192.6±10.1 mL/100mg/min，右侧 195±9.5 mL/100mg/min）明显高于 WT 兔子（左侧 99.2±29.1 mL/100mg/min，右侧 96.2±21.4 mL/100mg/min），但 EOCS-SU 组（左侧 97.6±11.3 mL/100mg/min，右侧 99±7.4 mL/100mg/min）与 WT 兔子之间的脑血流无显著差异。到 42 天大时，EOCS 兔子的脑血流与 WT 兔子无显著差异。
结论
缝切除术消除了 EOCS 兔子在 25 天大时皮质表面异常增加的脑血流。这一发现有助于证明缝切除术限制了颅缝早闭相关的颅内压和脑血流异常。
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