Han Hye-Yeon, Kim Hyung Joon, Jeong Seung-Hwa, Kim Jiyeon, Jeong Sung-Hee, Kim Gyoo Cheon, Hwang Dae-Seok, Kim Uk-Kyu, Ryu Mi Heon
Department of Oral Pathology, School of Dentistry, Research Institute for Oral Biotechnology, Pusan National University, Yangsan, Gyeongnam 50612, Republic of Korea.
Department of Oral Physiology, BK21 Plus Project and Institute of Translational Dental Sciences, School of Dentistry, Pusan National University, Yangsan, Gyeongnam 50612, Republic of Korea.
Evid Based Complement Alternat Med. 2018 May 13;2018:5765047. doi: 10.1155/2018/5765047. eCollection 2018.
Jaceosidin is a single compound from the Japanese mugwort , which is used as a food and a traditional medicinal herb. extracts and flavonoid components have been shown to have antihyperglycaemic, antioxidant, and anti-inflammatory properties. Although the anticancer properties of these extracts were recently demonstrated, the related mechanisms have not been characterised. In this study, we investigated the effects of jaceosidin in oral squamous cell carcinoma (OSCC) cells and initially showed selective suppression of proliferation (IC = 82.1 M in HSC-3 cells and 97.5 M in Ca9.22 cells) and accumulation of cells at the sub-G1 stage of the cell cycle. In addition, jaceosidin increased cleavage of caspase-9 and caspase-3 in OSCC cells, although caspase-8 was not detected. In further experiments, jaceosidin downregulated Akt phosphorylation and ectopic activation of Akt blocked the antiproliferative effects of jaceosidin. Finally, we showed that jaceosidin has no effects on HaCaT normal epithelial cell viability, indicating selective chemotherapeutic potential of jaceosidin and that tumour-specific downregulation of Akt increases apoptosis and inhibits growth in OSCC cells.
紫花前胡苷是从日本艾草中提取的单一化合物，日本艾草既是一种食物，也是一种传统草药。其提取物和黄酮类成分已被证明具有降血糖、抗氧化和抗炎特性。尽管最近已证实这些提取物具有抗癌特性，但其相关机制尚未明确。在本研究中，我们研究了紫花前胡苷对口腔鳞状细胞癌（OSCC）细胞的影响，初步显示其对细胞增殖有选择性抑制作用（在HSC-3细胞中IC = 82.1μM，在Ca9.22细胞中为97.5μM），并使细胞在细胞周期的G1期前阶段积累。此外，紫花前胡苷增加了OSCC细胞中caspase-9和caspase-3的裂解，尽管未检测到caspase-8。在进一步的实验中，紫花前胡苷下调了Akt磷酸化，而异位激活Akt则阻断了紫花前胡苷的抗增殖作用。最后，我们表明紫花前胡苷对HaCaT正常上皮细胞活力没有影响，这表明紫花前胡苷具有选择性化疗潜力，并且Akt的肿瘤特异性下调会增加OSCC细胞的凋亡并抑制其生长。
