Yakubu Yusuf, Shuaibu Abdulmalik Bello, Ibrahim Aliyu Musawa, Hassan Ummukulthum Lawal, Nwachukwu Raymond Junior
Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria.
Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria.
J Pathog. 2018 May 15;2018:8938597. doi: 10.1155/2018/8938597. eCollection 2018.
O157:H7 is an enteric foodborne pathogen associated with life threatening disease conditions. The enterobacteria are frequently found in cattle gastrointestinal tract with high potential of contaminating animal products such as meat, milk, and cheese. A cross-sectional study was conducted to investigate the presence of Shiga toxin-producing O157:H7 in milk products sold within Sokoto metropolis. Two hundred and sixty (260) samples (comprising 160 raw and 100 fermented milk samples) were collected from different sources within the study area. Bacteriological isolation and biochemical characterization yielded with a detection rate of 9.23% (24/260). Molecular identification of the recovered isolates by PCR amplification of the gene revealed O157:H7 with a positive rate of 20.83% (5/24). The overall prevalence of O157:H7 was 1.92% (5/260) and the positive proportions for raw and fermented milk samples were 1.86% (3/160) and 2.0% (2/100), respectively. Fisher's Exact test showed a nonsignificant association between the isolates and the different milk types ( = 0.943; OR = 0.94; [95% CI: 0.154-5.704]). The results revealed presence of O157:H7 in raw and fermented milk sold within Sokoto metropolis, Nigeria. The findings indicate possible feacal contamination of the milk products, with serious public health consequences. This necessitates the need to screen other milk products produced in the area such as butter and cheese. Health authorities in the State need to enlighten dairy farmers on the zoonotic potential of O157:H7 and the role of cattle in the spread of the pathogen.
O157:H7是一种与危及生命的疾病状况相关的食源性病原体。肠杆菌科细菌经常在牛的胃肠道中发现，具有污染肉类、牛奶和奶酪等动物产品的高可能性。开展了一项横断面研究，以调查在索科托市销售的乳制品中产生志贺毒素的O157:H7的存在情况。从研究区域内的不同来源收集了260份样本（包括160份生牛奶样本和100份发酵牛奶样本）。细菌学分离和生化特性鉴定的检出率为9.23%（24/260）。通过对该基因进行PCR扩增对回收的分离株进行分子鉴定，发现O157:H7的阳性率为20.83%（5/24）。O157:H7的总体患病率为1.92%（5/260），生牛奶样本和发酵牛奶样本的阳性比例分别为1.86%（3/160）和2.0%（2/100）。费舍尔精确检验显示分离株与不同类型牛奶之间无显著关联（P = 0.943；OR = 0.94；[95%置信区间：0.154 - 5.704]）。结果显示在尼日利亚索科托市销售的生牛奶和发酵牛奶中存在O157:H7。这些发现表明乳制品可能受到粪便污染，具有严重的公共卫生后果。这就需要对该地区生产的其他乳制品如黄油和奶酪进行筛查。该州的卫生当局需要向奶农宣传O157:H7的人畜共患病潜力以及牛在病原体传播中的作用。
