犬源性 和 移位检测。
-Shift Detection of Dog-Derived and .
机构信息
Guangdong Provincial Zoonosis Prevention and Control Key Laboratory, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510542, China.
Animal Science Department, Kano University of Science and Technology, Wudil, PMB 3244, Kano 20027, Nigeria.
出版信息
Biomed Res Int. 2018 May 13;2018:7617094. doi: 10.1155/2018/7617094. eCollection 2018.
To develop a -shift method for detection of dog-derived and three sets of primers were designed based on three SNPs (ITS71, ITS197, and ITS296) of their internal transcribed spacer 1 (ITS1) sequences. The detection effect of the -shift was assessed through the stability, sensitivity, accuracy test, and clinical detection. The results showed that these three sets of primers could distinguish accurately between and . The coefficient of variation in their values on the three SNPs was 0.09% and 0.15% (ITS71), 0.18% and 0.14% (ITS197), and 0.13% and 0.07% (ITS296), respectively. The lowest detectable concentration of standard plasmids for and was 5.33 × 10 ng/L and 5.03 × 10 ng/L. The -shift results of ten DNA samples from the dog-derived hookworms were consistent with their known species. In the clinical detection of 50 fecal samples from stray dogs, the positive rate of hookworm detected by -shift (42%) was significantly higher than that by microscopic examination (34%), and the former can identify the species. It is concluded that the -shift method is rapid, specific, sensitive, and suitable for the clinical detection and zoonotic risk assessment of the dog-derived hookworm.
为了开发一种用于检测犬源性 和 的 -shift 方法，我们基于其内部转录间隔区 1（ITS1）序列中的三个 SNP（ITS71、ITS197 和 ITS296）设计了三组引物。通过稳定性、灵敏度、准确性测试和临床检测评估了 -shift 的检测效果。结果表明，这三组引物能够准确地区分 和 。在三个 SNP 上，它们的 C 值的变异性分别为 0.09%和 0.15%（ITS71）、0.18%和 0.14%（ITS197）以及 0.13%和 0.07%（ITS296）。 和 的标准质粒的最低可检测浓度分别为 5.33×10ng/L 和 5.03×10ng/L。来自犬源钩虫的十个 DNA 样本的 -shift 结果与其已知物种一致。在对 50 份流浪狗粪便样本的临床检测中， -shift 检测到的钩虫阳性率（42%）显著高于显微镜检查（34%），前者可以识别 物种。综上所述，-shift 方法快速、特异、敏感，适用于犬源性钩虫的临床检测和人畜共患病风险评估。
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