Poniewierska-Baran Agata, Rajewska-Majchrzak Justyna, Ratajczak Mariusz Z
Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, Louisville, KY.
Department of Physiology, Pomeranian Medical University in Szczecin, Szczecin, Poland.
J Cancer Stem Cell Res. 2017;5. Epub 2017 Apr 27.
The erythropoietin receptor (EpoR) is expressed by cells from the erythroid lineage; however, evidence has accumulated that it is also expressed by some other non-hematopoietic tissues including several solid tumor cells and proposed candidates for cancer stem cells. This is an important concern, because recombinant erythropoietin (EPO) is frequently employed in cancer patients as a drug to ameliorate anemia related to chemo/radiotherapy. In our studies, we employed three human neuroblastoma (NB) cell lines and found in all of them the expression of EpoR and EPO mRNA. The functionality of EpoR in RMS cell lines was evaluated by chemotaxis, adhesion, and direct cell proliferation assays. We noticed that all three human NB cell lines responded to EPO stimulation by enhanced chemotaxis and cell adhesion. However, at the same time we did not observe any significant effect of EPO on proliferation. Based on this EPO supplementation in NB patients employed because of radio/chemotherapy induced anemias may have an unwanted side effect on tumor metastasis.
促红细胞生成素受体（EpoR）由红系谱系的细胞表达；然而，越来越多的证据表明，它也在一些其他非造血组织中表达，包括几种实体瘤细胞以及癌症干细胞的潜在候选细胞。这是一个重要问题，因为重组促红细胞生成素（EPO）在癌症患者中经常被用作药物来改善与化疗/放疗相关的贫血。在我们的研究中，我们使用了三种人神经母细胞瘤（NB）细胞系，并且在所有细胞系中都发现了EpoR和EPO mRNA的表达。通过趋化性、黏附以及直接细胞增殖试验评估了EpoR在横纹肌肉瘤（RMS）细胞系中的功能。我们注意到，所有三种人NB细胞系对EPO刺激的反应都是趋化性增强和细胞黏附增加。然而，与此同时，我们没有观察到EPO对增殖有任何显著影响。基于此，因放疗/化疗引起的贫血而对NB患者补充EPO可能会对肿瘤转移产生不良副作用。
