Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Chem Commun (Camb). 2018 Jun 19;54(50):6939-6942. doi: 10.1039/c8cc02451k.
Si-Rhodamines are bright fluorophores with red to near-infrared (NIR) emission, and are widely used for fluorescence imaging of biological phenomena. Here, in order to extend the scope of Si-rhodamine fluorophores, we established a versatile synthesis of unsymmetrical Si-rhodamines. To illustrate its value, we used one of these new fluorophores to synthesize a far-red to NIR fluorescence probe for hypoxia, and showed that it can visualize hepatic ischemia in mice in vivo.
硅罗丹明是一类具有红色到近红外（NIR）发射的明亮荧光染料，广泛用于生物现象的荧光成像。在此，为了扩展硅罗丹明荧光染料的应用范围，我们建立了一种通用的不对称硅罗丹明合成方法。为了说明其价值，我们使用其中一种新型荧光染料合成了一种用于缺氧的远红到近红外荧光探针，并证明其可在体可视化小鼠肝缺血。
