纳米盘中的脂质交换揭示细胞色素 P450 还原酶的膜边界。
Lipid-exchange in nanodiscs discloses membrane boundaries of cytochrome-P450 reductase.
机构信息
Biophysics and Department of Chemistry, University of Michigan, Ann Arbor, MI 48109-1055, USA.
出版信息
Chem Commun (Camb). 2018 Jun 14;54(49):6336-6339. doi: 10.1039/c8cc02003e.
Abstract
Lipids are critical for the function of membrane proteins. NADPH-cytochrome-P450-reductase, the sole electron transferase for microsomal oxygenases, possesses a conformational dynamics entwined with its topology. Here, we use peptide-nanodiscs to unveil cytochrome-P450-reductase's lipid boundaries, demonstrating a protein-driven enrichment of ethanolamine lipids (by 25%) which ameliorates by 3-fold CPR's electron-transfer ability.
摘要
脂质对于膜蛋白的功能至关重要。NADPH-细胞色素 P450 还原酶是细胞色素 P450 氧化酶的唯一电子转移酶，具有与其拓扑结构交织在一起的构象动力学。在这里，我们使用肽纳米盘来揭示细胞色素 P450 还原酶的脂质边界，证明了蛋白质驱动的乙醇胺脂质富集（增加了 25%），这使 CPR 的电子转移能力提高了 3 倍。
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