长链非编码 RNA CCAT1 促进前列腺癌细胞 PC-3 的迁移和侵袭。
Long non-coding RNA CCAT1 promotes the migration and invasion of prostate cancer PC-3 cells.
机构信息
Department of Urology Surgery, The First Hospital of Jiaxing, Jiaxing, China.
出版信息
Eur Rev Med Pharmacol Sci. 2018 May;22(10):2991-2996. doi: 10.26355/eurrev_201805_15055.
OBJECTIVE
To investigate the effect of long non-coding RNA CCAT1 on the proliferation, migration, and invasion of prostate cancer PC-3 cells.
PATIENTS AND METHODS
The expression of CCAT1 was detected by Real-time PCR. The effect of CCAT1 down-regulation on the proliferation of PC-3 cells was observed by MTT assay. The regulatory of CCAT1 low-expression on the migration ability of PC-3 cells was investigated by transwell assay. The influence of decreased CCAT1 on the invasion ability of PC-3 cells was detected by Matrigel invasion assay.
RESULTS
Increased CCAT1 was significantly related to lymph node metastasis in prostate cancer. Low-expression of CCAT1 could suppress cell proliferation. Knockdown of CCAT1 inhibited the migration of PC-3 cells. Down-regulation of CCAT1 attenuated the invasion of PC-3 cells.
CONCLUSIONS
CCAT1 promoted the growth and the metastasis of prostate cancer. Our findings might provide a potential target for the diagnosis and treatment of prostate cancer.
目的
探讨长链非编码 RNA CCAT1 对前列腺癌细胞 PC-3 增殖、迁移和侵袭的影响。
患者与方法
采用实时 PCR 检测 CCAT1 的表达。通过 MTT assay 观察 CCAT1 下调对 PC-3 细胞增殖的影响。通过 Transwell assay 研究 CCAT1 低表达对 PC-3 细胞迁移能力的调节作用。通过 Matrigel 侵袭 assay 检测降低 CCAT1 对 PC-3 细胞侵袭能力的影响。
结果
CCAT1 的增加与前列腺癌的淋巴结转移显著相关。CCAT1 低表达可抑制细胞增殖。敲低 CCAT1 抑制 PC-3 细胞的迁移。下调 CCAT1 减弱了 PC-3 细胞的侵袭。
结论
CCAT1 促进了前列腺癌的生长和转移。我们的研究结果可能为前列腺癌的诊断和治疗提供了一个潜在的靶点。
Zotero 插件