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[表面受体重新分布过程中淋巴细胞纤维成分的电子显微镜分析]

[Electron microscopic analysis of lymphocyte fibrillar elements during the redistribution of surface receptors].

作者信息

Bobrova I F, Komissarchik Ia Iu, Pinaev G P, Khomenko A V

出版信息

Tsitologiia. 1985 Mar;27(3):282-6.

PMID:2986331
Abstract

Filaments 5 nm thick, located throughout the cytoplasm mainly along the surface, are observed in intact lymphocytes. In the control glycerinized lymphocytes, besides the above filaments aggregations of filaments nearly 3 nm in diameter were found. After the treatment of cells by antimurine serum or ferritin-conjugated concanavalin A, some fibrillar structures are observed mainly in the cap region in the form of filaments 5-6 nm of thickness, radially directed towards the plasma membrane. After glycerinization, three types of filaments are observed being, respectively, near 3, 5-6 and almost 8 nm in diameter. Two latter types of filaments are decorated by S1-myosine fragments which indicates their actine nature. Differences in the character and distribution of myofibrils in the cytoplasm of intact cells and cells with caps may witness in favour of their involvement in the processes associated with redistribution of surface receptors.

摘要

在完整的淋巴细胞中观察到直径为5纳米的细丝,主要沿表面分布于整个细胞质中。在对照甘油处理的淋巴细胞中,除上述细丝外,还发现了直径近3纳米的细丝聚集体。用抗鼠血清或铁蛋白结合的伴刀豆球蛋白A处理细胞后,主要在帽区观察到一些纤维状结构,呈厚度为5 - 6纳米的细丝形式,径向指向质膜。甘油处理后,观察到三种类型的细丝,直径分别约为3、5 - 6和近8纳米。后两种类型的细丝被S1 - 肌球蛋白片段标记,这表明它们具有肌动蛋白性质。完整细胞和有帽细胞细胞质中肌原纤维的特征和分布差异可能表明它们参与了与表面受体重新分布相关的过程。

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