Nalbandian Minas, Radak Zsolt, Takeda Masaki
Graduate School of medicine, Kyoto University;
Research Institute of Sports Science, University of Physical Education.
J Vis Exp. 2018 May 18(135):56890. doi: 10.3791/56890.
The measurement of metabolic and endocrinal markers during physical activity is of relevance to understanding the physiological implications of different exercise modalities. During some exercise modalities (e.g., high-intensity interval exercise), blood metabolites and hormonal levels change in short periods of time. In the present study, we describe a method to catheterize the antecubital vein, which allows the collection of several blood samples during exercise. Insulin and venous lactate concentrations were measured during high-intensity exercise by the application of the described method. The exercise consisted of three 30 s bouts of high-intensity exercise separated by 4 min of recovery. After the last recovery period, a Wingate test was performed. Blood samples from the antecubital vein were obtained before and after each 30 s bout and before and after the Wingate test. As a result, it was possible to evaluate the plasma insulin and venous blood lactate variations during the exercise.
在体育活动期间测量代谢和内分泌标志物，对于理解不同运动方式的生理意义具有重要意义。在某些运动方式（如高强度间歇运动）中，血液代谢物和激素水平会在短时间内发生变化。在本研究中，我们描述了一种经肘前静脉插入导管的方法，该方法可在运动期间采集多个血样。通过应用所描述的方法，在高强度运动期间测量了胰岛素和静脉血乳酸浓度。运动由三次30秒的高强度运动组成，每次运动之间有4分钟的恢复时间。在最后一次恢复阶段后，进行了一次温盖特测试。在每次30秒运动前后以及温盖特测试前后，从肘前静脉采集血样。结果，得以评估运动期间血浆胰岛素和静脉血乳酸的变化。
