Fransson L A, Del Rosso M
Acta Chem Scand B. 1985;39(3):163-70. doi: 10.3891/acta.chem.scand.39b-0163.
35S-Labelled heparan sulfates derived from the culture medium (extracellular), a trypsinate of the cells (pericellular) and the cell residue (intracellular) of quiescent normal, proliferating normal or SV40-transformed 3T3 cells were analyzed for charge heterogeneity, by ion exchange chromatography and for self-affinity, by chromatography on heparan sulfate-agarose gels. Quiescent normal cells retained most of their heparan sulphate intra- or pericellularly. The surface-exposed material was charge heterogeneous and had a strong affinity for heparan sulfate. In cultures of growing cells and transformed cells most of the heparan sulfate was found in the medium. The heparan sulfate retained on the surface or growing cells had a lower self-affinity than did the corresponding material from normal and transformed cells. Although cell surface heparan sulfates from transformed cells showed affinity for a matrix substituted with the total heparan sulfate pool, the affinity for one particular subtype was much less pronounced or non-existent.
对来源于静止的正常、增殖的正常或SV40转化的3T3细胞的培养基（细胞外）、细胞胰蛋白酶消化物（细胞周）和细胞残渣（细胞内）的35S标记硫酸乙酰肝素进行了分析，通过离子交换色谱分析其电荷异质性，通过硫酸乙酰肝素-琼脂糖凝胶色谱分析其自身亲和力。静止的正常细胞在细胞内或细胞周保留了大部分硫酸乙酰肝素。表面暴露的物质电荷不均一，对硫酸乙酰肝素有很强的亲和力。在生长细胞和转化细胞的培养物中，大部分硫酸乙酰肝素存在于培养基中。保留在生长细胞表面的硫酸乙酰肝素的自身亲和力低于正常细胞和转化细胞的相应物质。尽管来自转化细胞的细胞表面硫酸乙酰肝素对用总硫酸乙酰肝素池替代的基质表现出亲和力，但对一种特定亚型的亲和力则不太明显或不存在。
