Department of Microbiology, Faculty of Science, King Mongkut's University of Technology Thonburi (KMUTT), Bangkok, 10140, Thailand; Fish Health Platform, Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol University, 272 Rama VI Road, Bangkok, 10400, Thailand.
Fish Health Platform, Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol University, 272 Rama VI Road, Bangkok, 10400, Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, 12120, Thailand.
Microb Pathog. 2018 Sep;122:46-52. doi: 10.1016/j.micpath.2018.06.005. Epub 2018 Jun 2.
The Siamese fighting fish (Betta splendens) is one of the popular aquarium ornamental fish in the global trade. Large numbers of ornamental fish farmed in central Thailand suffered from two common syndromes; preliminarily named skin nodule syndrome (SNS) and big belly syndrome (BBS): they showed noticeable clinical signs of abnormal appearances resulting in depressed saleability. Since very few specifics are known about causative agents of these syndromes, this study aimed at investigating histopathological features and culturable bacteria associated with these fish infected in the process of farming. Histopathologically, SNS fish consistently exhibited necrosis and severe melanization in the muscles and multiple internal organs. Whereas BBS fish exhibited either typical granulomas or tissue damage associated with acid-fast stained bacteria and Gram negative bacteria, respectively. Six different Gram negative bacterial species were recovered from BBS fish while 23 bacterial species belonging to 14 genera were recovered from fish suffering from SNS. Most of the culturable bacteria are new to betta fish and some of them are known to be marine bacteria, suggesting possible entry route via a contaminated live feed, commercial Artemia shrimp. The true causative agents of these syndromes remain unclear. However, histopathological changes and existence of a wide range of bacteria associated with the naturally diseased fish suggest involvement of multiple bacterial infections.
暹罗斗鱼（Betta splendens）是全球贸易中受欢迎的观赏鱼类之一。大量在泰国中部养殖的观赏鱼患有一种常见的两种综合征；初步命名为皮肤结节综合征（SNS）和大肚子综合征（BBS）：它们表现出明显的异常外观临床症状，导致销售不畅。由于对这些综合征的病原体知之甚少，因此本研究旨在调查与养殖过程中感染这些鱼类的相关组织病理学特征和可培养细菌。组织病理学上，SNS 鱼类的肌肉和多个内部器官始终表现出坏死和严重的黑化。而 BBS 鱼类则表现出典型的肉芽肿或与抗酸染色细菌和革兰氏阴性菌相关的组织损伤。从 BBS 鱼类中分离出六种不同的革兰氏阴性细菌，而从患有 SNS 的鱼类中分离出 23 种细菌，属于 14 个属。从这些患病鱼中分离出的大多数可培养细菌都是新的斗鱼鱼属细菌，其中一些是已知的海洋细菌，这表明它们可能是通过受污染的活饲料（商业丰年虾）进入鱼体内的。这些综合征的真正病原体仍不清楚。然而，与自然患病鱼类相关的组织病理学变化和广泛存在的细菌表明存在多种细菌感染。
