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大鼠肠上皮细胞的基底外侧膜：从刷状缘污染中纯化该膜。

The basolateral membrane of rat enterocyte: its purification from brush border contamination.

作者信息

Orsenigo M N, Tosco M, Esposito G, Faelli A

出版信息

Anal Biochem. 1985 Feb 1;144(2):577-83. doi: 10.1016/0003-2697(85)90156-3.

DOI:10.1016/0003-2697(85)90156-3
PMID:2986482
Abstract

Basolateral membranes obtained by self-orienting Percoll-gradient centrifugation were treated with 5 mM CaCl2 to minimize the cross-contamination by brush border membranes. From marker enzyme-specific activities it was calculated that in this preparation the basolateral/brush border membrane ratio was 22.6. A low L-glucose permeability across basolateral membrane vesicles together with ATP-dependent sodium uptake was observed.

摘要

通过自我定向Percoll梯度离心获得的基底外侧膜用5 mM氯化钙处理，以尽量减少刷状缘膜的交叉污染。根据标记酶的比活性计算得出，在该制剂中基底外侧膜与刷状缘膜的比例为22.6。观察到基底外侧膜囊泡对L-葡萄糖的低通透性以及ATP依赖的钠摄取。

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