Zalisko Edward J, Larsen John H
Department of Zoology and Electron Microscopy Center, Washington State University, Pullman, Washington 99164-4220.
J Morphol. 1990 Mar;203(3):321-330. doi: 10.1002/jmor.1052030307.
The vas deferens of Ambystoma macrodactylum is composed of a peritoneal epithelium, connective tissue layer with fibroblasts, circular smooth muscle, capillaries, cells containing lipid, and a luminal epithelium composed of a single layer of cuboidal cells covered by a net of interconnected ciliated squamous cells. The cuboidal cells have abundant rough endoplasmic reticulum, mitochondria, and PAS + secretory vesicles. Squamous cells of breeding males consistently have tufts of ∼100 cilia located at one end of the long axis of each cell. These cilia may help distribute secretory products. The squamous cells, absent in post-breeding males, are apparently sloughed into the lumen. Lipid vesicles are present throughout the cytoplasm of the cuboidal and squamous epithelial cells and are also in some cells of the connective tissue layer. These vesicles increase dramatically in number during the first 4 weeks after breeding and may serve as an energy pool for the next breeding season. Enzyme-histochemical tests for testosterone synthesis were negative. In addition to the accumulation of lipid and the loss of squamous cells in the vas deferens, after breeding PAS + vesicle production is terminated. These alterations appear to represent energy conservation strategies employed by the sperm-depleted vas deferens.
大趾钝口螈的输精管由腹膜上皮、含有成纤维细胞的结缔组织层、环形平滑肌、毛细血管、含脂质细胞以及由单层立方细胞组成的管腔上皮构成，单层立方细胞被相互连接的纤毛鳞状细胞网覆盖。立方细胞有丰富的粗面内质网、线粒体和PAS阳性分泌小泡。繁殖期雄性的鳞状细胞在每个细胞长轴的一端始终有一束约100根的纤毛。这些纤毛可能有助于分泌产物的分布。繁殖期后的雄性体内不存在鳞状细胞，显然已脱落至管腔中。脂质小泡存在于立方上皮细胞和鳞状上皮细胞的整个细胞质中，结缔组织层的一些细胞中也有。这些小泡在繁殖后的前4周数量急剧增加，可能作为下一个繁殖季节的能量储备。睾酮合成的酶组织化学检测呈阴性。除了输精管中脂质的积累和鳞状细胞的消失外，繁殖后PAS阳性小泡的产生也终止了。这些变化似乎代表了精子耗尽的输精管所采用的能量节约策略。
