Newton J F, Eckardt R, Bender P E, Leonard T, Straub K
Biochem Biophys Res Commun. 1985 Apr 30;128(2):733-8. doi: 10.1016/0006-291x(85)90108-1.
Leukotriene B4 was metabolized in rat hepatic microsomes to two products. Mass spectral analysis of these two metabolites indicated that the major metabolite was the 20-hydroxy metabolite while the minor metabolite was the 19-hydroxy metabolite. The formation of these metabolites required NADPH and was linear with time (20 min) and protein (1.6 mg/ml). The Km apparent and Vmax for omega hydroxylation of LTB4 was 14 uM and 0.138 nmol/min/mg protein. In contrast, the km and Vmax for omega minus one hydroxylation was 54 uM and 0.093 nmol/min/mg protein. These results suggest that omega and omega minus one hydroxylations of LTB4 may be mediated by different isozymes of hepatic P-450.
白三烯B4在大鼠肝微粒体中代谢为两种产物。对这两种代谢物的质谱分析表明，主要代谢物是20-羟基代谢物，而次要代谢物是19-羟基代谢物。这些代谢物的形成需要NADPH，并且与时间（20分钟）和蛋白质（1.6毫克/毫升）呈线性关系。LTB4 ω-羟基化的表观Km和Vmax分别为14微摩尔和0.138纳摩尔/分钟/毫克蛋白质。相比之下，ω-1羟基化的Km和Vmax分别为54微摩尔和0.093纳摩尔/分钟/毫克蛋白质。这些结果表明，LTB4的ω-和ω-1羟基化可能由肝脏P-450的不同同工酶介导。
