Esmann M
Biochim Biophys Acta. 1985 May 14;815(2):196-202. doi: 10.1016/0005-2736(85)90289-5.
Occlusion of Rb+ by C12E8-solubilized (Na+ + K+)-ATPase from shark salt glands has been measured. The rate of de-occlusion at room temperature is about 1 s-1, which is the same as for the membrane-bound enzyme. The amount of Rb+ occluded is 3 moles Rb+ per mole membrane-bound shark enzyme, whereas only about 2 moles Rb+ are occluded by the C12E8-solubilized enzyme.
已对来自鲨鱼盐腺的C12E8增溶的(Na⁺ + K⁺)-ATP酶对Rb⁺的封闭情况进行了测定。室温下的去封闭速率约为1 s⁻¹,这与膜结合酶的情况相同。每摩尔膜结合的鲨鱼酶封闭的Rb⁺量为3摩尔Rb⁺,而C12E8增溶的酶仅封闭约2摩尔Rb⁺。