二价阳离子对溶解的钠钾ATP酶的沉淀作用。
Precipitation of solubilized Na+/K+-ATPase by divalent cations.
作者信息
Esmann M
机构信息
Institute of Biophysics, University of Aarhus, Denmark.
出版信息
Biochim Biophys Acta. 1988 May 9;940(1):71-6. doi: 10.1016/0005-2736(88)90009-0.
PMID:2835103
Abstract
A method for preparation of membranous fragments of pure and highly active shark rectal gland Na+/K+-ATPase by Mn2+ precipitation of C12E8-solubilized enzyme is described. The method is rapid and inexpensive, and yields enzyme with a specific Na+/K+-ATPase activity of up to 1800 mumol/mg per h at 37 degrees C. The influence of the detergent/protein and lipid/protein ratios on the yield of membrane bound enzyme is described.
摘要
描述了一种通过用Mn2+沉淀C12E8增溶的酶来制备纯净且高活性的鲨鱼直肠腺Na+/K+-ATP酶膜片段的方法。该方法快速且成本低廉，在37摄氏度下可产生具有高达1800 μmol/mg每小时的特定Na+/K+-ATP酶活性的酶。还描述了去污剂/蛋白质和脂质/蛋白质比例对膜结合酶产量的影响。
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