Department of Oral and Maxillofacial Surgery, College and Hospital of Stomatology, Hebei Medical University; The Key Laboratory of Stomatology, Shijiazhuang, Hebei, China (mainland).
Department of Oral Pathology, College and Hospital of Stomatology, Hebei Medical University; Key Laboratory of Stomatology, Shijiazhuang, Hebei, China (mainland).
Med Sci Monit. 2018 Jun 5;24:3772-3781. doi: 10.12659/MSM.911014.
BACKGROUND Salivary pleomorphic adenoma is one of the most common salivary gland tumors. It has a relatively high tendency to recur and a high risk of malignant transformation. The present study aimed to study the effect of XT-I gene silencing on the implanting growth of salivary pleomorphic adenoma. MATERIAL AND METHODS Primary cultures of SPA cells and fibroblasts from the same patient were assessed. The adenovirus vector Ad-shRNA-XT-I was constructed and transfected into SPA cells. The expression of XT-I gene and XT-I protein was detected by real-time PCR and Western blot. The contents of proteoglycans were detected. The SPA cells transfected with Ad-shRNA-XT-I (group SPA-XT-I) and Ad-shRNA-HK (group SPA-HK), as well as without transfection (group SPA), were implanted into ADM scaffold with fibroblasts and then transferred into 18 BALB/C-nu nude mice for 3 months. RESULTS Primary cultures showed SPA cells were positive for human CK and S-100 protein and the fibroblasts were positive for human vimentin. The expressions of XT-I gene and protein were decreased by 51% and 51.31%, respectively. The content of proteoglycans was reduced by 48.45%. The results of the implanting growth in vitro and in vivo of nude mice indicated that no tumors grew in the SPA-XT-I group, whereas SPA grew in groups SPA-HK and SPA positive for human a-SMA, S-100 protein, and calponin. CONCLUSIONS XT-I gene silencing effectively inhibited the implanting growth of SPA.
唾液腺多形性腺瘤是最常见的唾液腺肿瘤之一，它具有相对较高的复发倾向和恶性转化风险。本研究旨在研究 XT-I 基因沉默对唾液腺多形性腺瘤种植生长的影响。
评估了来自同一患者的 SPA 细胞和成纤维细胞的原代培养物。构建了腺病毒载体 Ad-shRNA-XT-I 并转染 SPA 细胞。通过实时 PCR 和 Western blot 检测 XT-I 基因和 XT-I 蛋白的表达。检测糖胺聚糖的含量。转染 Ad-shRNA-XT-I（SPA-XT-I 组）和 Ad-shRNA-HK（SPA-HK 组）的 SPA 细胞以及未转染的 SPA 细胞（SPA 组）与成纤维细胞一起植入 ADM 支架中，然后转移到 18 只 BALB/C-nu 裸鼠中，共 3 个月。
原代培养显示 SPA 细胞呈人 CK 和 S-100 蛋白阳性，成纤维细胞呈人波形蛋白阳性。XT-I 基因和蛋白的表达分别降低了 51%和 51.31%。糖胺聚糖的含量减少了 48.45%。裸鼠体内外种植生长的结果表明，SPA-XT-I 组无肿瘤生长，而 SPA-HK 组和 SPA 组则生长了人 a-SMA、S-100 蛋白和钙调蛋白阳性的肿瘤。
XT-I 基因沉默有效抑制了 SPA 的种植生长。
