Hahn E F
Clin Exp Hypertens A. 1985;7(1):123-37. doi: 10.3109/10641968509074758.
The effect of 100 mM sodium chloride on the binding of [3H]-naltrexone to rat brain opiate receptors in spontaneously hypertensive (SHR) and normotensive (WKY) rats was studied. The percentage increase in binding in the presence of sodium chloride did not vary with age in WKY rats. Brain homogenates from 4 week old SHR rats incubated with 100 mM sodium chloride exhibited a similar increase in binding compared to age matched WKY rats. In contrast, brain preparations from 6, 14 and 20 week old SHR rats were more sensitive to sodium chloride, and the increase in binding of [3H]-naltrexone was significantly greater in these animals than in corresponding normotensive ones. Since blood pressure is increased in SHR rats compared to WKY rats at these ages, these results suggest that elevated blood pressure may be correlated with an increase in opiate receptor sensitivity to sodium chloride. The effect of in vivo sodium chloride was examined by feeding the animals a diet containing 4% salt. This concentration of salt did not significantly alter the binding of [3H]-naltrexone to rat brain homogenates prepared from 8 week old SHR rats. These results suggest that higher levels of sodium chloride and longer exposure to the diet may be required to observe the salt sensitivity produced by 100 mM salt in the in vitro radioreceptor assay.
研究了100 mM氯化钠对自发性高血压（SHR）大鼠和正常血压（WKY）大鼠脑阿片受体与[3H] -纳曲酮结合的影响。在WKY大鼠中，氯化钠存在时结合增加的百分比不随年龄变化。与年龄匹配的WKY大鼠相比，用100 mM氯化钠孵育的4周龄SHR大鼠的脑匀浆结合增加情况相似。相比之下，6周龄、14周龄和20周龄SHR大鼠的脑制剂对氯化钠更敏感，这些动物中[3H] -纳曲酮的结合增加显著大于相应的正常血压大鼠。由于在这些年龄SHR大鼠的血压高于WKY大鼠，这些结果表明血压升高可能与阿片受体对氯化钠的敏感性增加相关。通过给动物喂食含4%盐的饮食来检查体内氯化钠的作用。该盐浓度并未显著改变从8周龄SHR大鼠制备的大鼠脑匀浆中[3H] -纳曲酮的结合。这些结果表明，在体外放射受体测定中，可能需要更高水平的氯化钠和更长时间的饮食暴露才能观察到100 mM盐产生的盐敏感性。
