Kolena Y, Channing C P
Horm Res. 1985;21(3):185-98. doi: 10.1159/000180044.
Follicular fluid obtained from large (6-12 mm) porcine follicles (LFF) was investigated to determine its stimulatory activity on progesterone secretion and on follicle stimulating hormone (FSH) induction of 125I-human chorionic gonadotropin (hCG)-luteinizing hormone (LH) binding sites in porcine granulosa cells in a 4-day culture. Incubation of granulosa cells harvested from small porcine follicles (1-2 mm) with 50% LFF led to stimulation of LH/hCG binding sites and progesterone secretion. After partial purification of pooled LFF or proteins precipitated with 90% ethanol on Sephadex G-100 the greatest stimulatory activity was found in the second protein peak eluted from the column. Chromatography of part of the active fraction on DEAE Sephacel using a continuous gradient of NH4HCO3 yielded seven protein fractions. The second fraction, which eluted early, contained the majority of the stimulatory activity which was purified about 32-fold compared to native LFF. In contrast, addition of follicular fluid recovered from small porcine follicles inhibited FSH induction of LH/hCG binding sites and progesterone secretion. It can be concluded, that maturation of granulosa cells from small follicles may be enhanced by protein(s) present in LFF, but not in fluid recovered from less mature follicles.
对从大型（6 - 12毫米）猪卵泡中获取的卵泡液（LFF）进行了研究，以确定其在4天培养过程中对猪颗粒细胞中孕酮分泌以及促卵泡激素（FSH）诱导的125I - 人绒毛膜促性腺激素（hCG） - 促黄体生成素（LH）结合位点的刺激活性。将从小型猪卵泡（1 - 2毫米）收获的颗粒细胞与50%的LFF一起孵育，可刺激LH/hCG结合位点和孕酮分泌。在Sephadex G - 100上对合并的LFF或用90%乙醇沉淀的蛋白质进行部分纯化后，在从柱上洗脱的第二个蛋白峰中发现了最大的刺激活性。使用NH4HCO3的连续梯度在DEAE Sephacel上对部分活性馏分进行色谱分析，得到七个蛋白馏分。较早洗脱的第二个馏分含有大部分刺激活性，与天然LFF相比，其纯化倍数约为32倍。相反，添加从小型猪卵泡中回收的卵泡液会抑制FSH诱导的LH/hCG结合位点和孕酮分泌。可以得出结论，LFF中存在的蛋白质可能会增强小型卵泡颗粒细胞的成熟，但未成熟卵泡中回收的卵泡液中的蛋白质则不会。
