Azari Aala, Shokrzadeh Mohammad, Zamani Ehsan, Amani Nahid, Shaki Fatemeh
a Pharmaceutical Sciences Research Center , Mazandaran University of Medical Sciences , Sari , Iran.
b Department of Toxicology and Pharmacology, Faculty of Pharmacy , Mazandaran University of Medical Sciences , Sari , Iran.
Drug Chem Toxicol. 2019 Jan;42(1):54-59. doi: 10.1080/01480545.2018.1477793. Epub 2018 Jun 6.
Acrylamide (AA) is a toxic chemical compound found in cooked foods. Considerable evidences suggest that oxidative stress and mitochondrial dysfunction are contributed to AA toxicity. Ceric oxide (CeO) nanoparticles (nano-ceria) have the potential to be developed as a therapeutic for oxidative stress insults due to their catalytic antioxidant properties. In this study we investigated, whether nano-ceria exerted a protective effect against AA-induced cytotoxicity and oxidative damage. HepG2 human cancer cell lines were exposed to nano-ceria (50, 100, and 200 µM) and after 30 min, AA in the half maximal inhibitory concentration (IC50) concentration (200 µM) was added to the cells. Twenty four hours later, cellular viability, reactive oxygen species (ROS) generation, lipid peroxidation (LPO), and cellular levels of glutathione (GSH) were assayed. AA decreased cell viability and pretreatment with nano-ceria significantly decreased AA-induced cytotoxicity. In addition, nano-ceria alleviated AA-induced ROS generation and LPO and depressed GSH level. Our results suggested that nano-ceria prevented cellular and oxidative damage induced by AA.
丙烯酰胺（AA）是一种在熟食中发现的有毒化合物。大量证据表明，氧化应激和线粒体功能障碍与AA毒性有关。氧化铈（CeO）纳米颗粒（纳米氧化铈）因其催化抗氧化特性，有潜力被开发成为一种针对氧化应激损伤的治疗方法。在本研究中，我们调查了纳米氧化铈是否对AA诱导的细胞毒性和氧化损伤具有保护作用。将HepG2人癌细胞系暴露于纳米氧化铈（50、100和200μM）中，30分钟后，向细胞中加入半数最大抑制浓度（IC50）（200μM）的AA。24小时后，检测细胞活力、活性氧（ROS）生成、脂质过氧化（LPO）和细胞内谷胱甘肽（GSH）水平。AA降低了细胞活力，而纳米氧化铈预处理显著降低了AA诱导的细胞毒性。此外，纳米氧化铈减轻了AA诱导的ROS生成和LPO，并降低了GSH水平。我们的结果表明，纳米氧化铈可预防AA诱导的细胞和氧化损伤。
