Research Laboratory of Comparative Biochemistry and Molecular Biology, Ternopil National Pedagogical University, Ternopil, Ukraine.
Research Laboratory of Comparative Biochemistry and Molecular Biology, Ternopil National Pedagogical University, Ternopil, Ukraine; Department of General Chemistry, Ternopil State Medical University, Ternopil, Ukraine.
Chemosphere. 2018 Feb;193:1127-1142. doi: 10.1016/j.chemosphere.2017.11.079. Epub 2017 Nov 17.
Bivalve mollusks from the cooling reservoirs of fuel power plants (PP) are acclimated to the chronic heating and chemical pollution. We investigated stress responses of the mussels from these ponds to determine their tolerance to novel environmental pollutant, zinc oxide nanoparticles (nZnO). Male Unio tumidus from the reservoirs of Dobrotvir and Burschtyn PPs (DPP and BPP), Ukraine were exposed for 14 days to nZnO (3.1 μM), Zn (3.1 μM) at 18 °C, elevated temperature (T, 25 °C), or nZnO at 25 °C (nZnO + T). Control groups were held at 18 °C. Zn-containing exposures resulted in the elevated concentrations of total and Zn-bound metallothionein (MT and Zn-MT) in the digestive gland, an increase in the levels of non-metalated MT (up to 5 times) and alkali-labile phosphates and lysosomal membrane destabilization in hemocytes. A common signature of nZnO exposures was modulation of the multixenobiotic-resistance protein activity (a decrease in the digestive gland and increase in the gills). The origin of population strongly affected the cellular stress responses of mussels. DPP-mussels showed depletion of caspase-3 in the digestive gland and up-regulation of HSP70, HSP72 and HSP60 levels in the gill during most exposures, whereas in the BPP-mussels caspase-3 was up-regulated and HSPs either downregulated or maintained stable. BPP-mussels were less adapted to heating shown by a glutathione depletion at elevated temperature (25 °C). Comparison with the earlier studies on mussels from pristine habitats show that an integrative 'eco-exposome'-based approach is useful for the forecast of the biological responses to novel adverse effects on aquatic organisms.
来自燃料发电厂（PP）冷却水库的双壳类软体动物适应了慢性加热和化学污染。我们研究了这些池塘中的贻贝对新型环境污染物氧化锌纳米颗粒（nZnO）的应激反应，以确定它们对新型环境污染物的耐受性。来自乌克兰 Dobrotvir 和 Burschtyn PP（DPP 和 BPP）水库的雄性 Unio tumidus 在 18°C 下暴露于 nZnO（3.1 μM）、Zn（3.1 μM）、高温（T，25°C）或 25°C 下的 nZnO（nZnO+T）14 天。对照组保持在 18°C。含 Zn 的暴露导致消化腺中总金属硫蛋白（MT 和 Zn-MT）和非金属化 MT 水平升高（高达 5 倍）以及碱性不稳定磷酸酯和溶酶体膜不稳定，血液细胞中的金属硫蛋白（MT 和 Zn-MT）。nZnO 暴露的共同特征是多药物耐药蛋白活性的调节（消化腺减少，鳃增加）。种群的起源强烈影响贻贝的细胞应激反应。DPP 贻贝在大多数暴露中显示消化腺中 caspase-3 耗竭和鳃中 HSP70、HSP72 和 HSP60 水平上调，而 BPP 贻贝中 caspase-3 上调，HSPs 下调或维持稳定。BPP 贻贝对加热的适应能力较差，在高温（25°C）时谷胱甘肽耗竭。与先前对来自原始生境的贻贝的研究相比，基于综合“生态暴露组”的方法可用于预测水生生物对新型不利影响的生物学反应。
