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二丁酰环磷腺苷诱导性碱性磷酸酶水平改变的突变型L-M细胞的生化与免疫学特性

Biochemical and immunological characterization of mutant L-M cells with altered levels of dibutyryl cyclic AMP-inducible alkaline phosphatase.

作者信息

Gum J R, Raetz C R

出版信息

Mol Cell Biol. 1985 May;5(5):1184-7. doi: 10.1128/mcb.5.5.1184-1187.1985.

DOI:10.1128/mcb.5.5.1184-1187.1985
PMID:2987678
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC366837/
Abstract

Phosphotyrosine-Sepharose 4B was synthesized and used to purify L-cell alkaline phosphatase. Antibodies to this enzyme interacted with the alkaline phosphatase of strains A-1-2 and A-3-3, mutants that express the enzyme constitutively. This and thermal stability studies suggest that these mutants contain the same alkaline phosphatase isozyme as their parent strain.

摘要

合成了磷酸酪氨酸琼脂糖凝胶4B，并用于纯化L细胞碱性磷酸酶。针对这种酶的抗体与A-1-2和A-3-3菌株的碱性磷酸酶相互作用，这两种菌株是组成型表达该酶的突变体。这一点以及热稳定性研究表明，这些突变体与其亲本菌株含有相同的碱性磷酸酶同工酶。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1aaf/366837/6aa7c8a91318/molcellb00101-0288-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1aaf/366837/3bcd47581e2b/molcellb00101-0287-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1aaf/366837/6aa7c8a91318/molcellb00101-0288-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1aaf/366837/3bcd47581e2b/molcellb00101-0287-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1aaf/366837/6aa7c8a91318/molcellb00101-0288-a.jpg

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本文引用的文献

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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Adenosine 3',5'-phosphate in biological materials. I. Purification and properties of cyclic 3',5'-nucleotide phosphodiesterase and use of this enzyme to characterize adenosine 3',5'-phosphate in human urine.生物材料中的3',5'-磷酸腺苷。I. 环3',5'-核苷酸磷酸二酯酶的纯化及性质，以及用该酶鉴定人尿中的3',5'-磷酸腺苷
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Production of antisera with small doses of immunogen: multiple intradermal injections.
用小剂量免疫原制备抗血清：多次皮内注射。
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Partial characterization of two mouse liver alkaline phosphatases that require manganese for activity.两种需要锰来激活的小鼠肝脏碱性磷酸酶的部分特性分析。
Int J Biochem. 1982;14(11):977-81. doi: 10.1016/0020-711x(82)90058-1.
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Human liver acid phosphatases: purification and properties of a low-molecular-weight isoenzyme.人肝脏酸性磷酸酶：一种低分子量同工酶的纯化及性质
Arch Biochem Biophys. 1982 Apr 1;214(2):505-15. doi: 10.1016/0003-9861(82)90054-6.
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Biosynthesis of the erythrocyte anion transport protein.红细胞阴离子转运蛋白的生物合成
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Convenient selection of monoclonal antibodies to isoenzymes of placental alkaline phosphatase using the catalytic activity of the antigen in enzyme-antigen immunoassay.利用酶-抗原免疫测定中抗原的催化活性，方便地筛选胎盘碱性磷酸酶同工酶的单克隆抗体。
Anal Biochem. 1982 Aug;124(2):286-92. doi: 10.1016/0003-2697(82)90040-9.
8
Dibutyryl cAMP-inducible alkaline phosphatase in animal cell plasma membranes: fluorescence detection of mutant clones on polyester cloth.动物细胞质膜中丁酰化环磷腺苷诱导的碱性磷酸酶：聚酯布上突变克隆的荧光检测
Proc Natl Acad Sci U S A. 1983 Jul;80(13):3918-22. doi: 10.1073/pnas.80.13.3918.
9
Forskolin: unique diterpene activator of adenylate cyclase in membranes and in intact cells.福斯高林：膜及完整细胞中独特的腺苷酸环化酶二萜激活剂。
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3363-7. doi: 10.1073/pnas.78.6.3363.
10
The cyclic AMP-mediated induction of alkaline phosphatase in mouse L-cells.环磷酸腺苷介导的小鼠L细胞碱性磷酸酶诱导作用
J Biol Chem. 1981 Feb 10;256(3):1396-403.