Cao Famin, Qi Jianhong, Song Hongqiang, Xie Di, Zhou Lu, Han Yunning, Li Hao, Wu Qi, Dong Jun, Zhang Yanming
Institute of Sports Medicine, Taishan Medical University, 619 Great Wall Road, Tai'an, 271016, Shandong, China.
Institute of Sports Medicine, Taishan Medical University, 619 Changcheng Road, Tai'an, 271016, Shandong, China.
Cell Tissue Bank. 2018 Dec;19(4):549-558. doi: 10.1007/s10561-018-9703-4. Epub 2018 Jun 6.
To compare the effects of Tsmu solution with vitrification on chondrocyte viability and examine histological and biomechanical properties of osteochondral allografts (OCAs) after storage, OCAs from femoral condyles of New Zealand rabbits were harvested, stored for 35 days in Tsmu solution or by in vitro vitrification, and subjected to in vivo and in vitro assays. Stored OCAs were transplanted into knee femoral condyle cartilage defects in recipient rabbits. Chondrocyte viability and histological changes of cartilage grafts were assessed in vitro. Gross assessment, chondrocyte viability, histological assessment, OCA biomechanics, and immunological markers were evaluated in vivo 6 months after transplantation. Fresh OCAs served as in vitro and in vivo controls. Chondrocyte viability and scores for cartilage surface and histological quantitative assessment were superior for Tsmu solution compared with vitrification, but inferior compared with fresh OCAs in vitro and in vivo. With the exception of interleukin 6 content, biomechanical features of samples stored in Tsmu solution were superior to vitrification, and inferior to fresh OCAs in vivo. Thus, Tsmu solution provided suitable storage that improved chondrocyte viability, intact OCA cartilage matrix architecture, and transplantation outcomes.
为比较四甲基脲（Tsmu）溶液与玻璃化保存对软骨细胞活力的影响，并检测储存后同种异体骨软骨移植体（OCAs）的组织学和生物力学特性，从新西兰兔股骨髁获取OCAs，分别在Tsmu溶液中或通过体外玻璃化保存35天，然后进行体内和体外检测。将储存后的OCAs移植到受体兔膝关节股骨髁软骨缺损处。体外评估软骨移植体的软骨细胞活力和组织学变化。移植后6个月，在体内评估大体情况、软骨细胞活力、组织学评估、OCA生物力学及免疫标志物。新鲜OCAs作为体外和体内对照。与玻璃化保存相比，Tsmu溶液保存的软骨细胞活力以及软骨表面评分和组织学定量评估结果更优，但在体内和体外均低于新鲜OCAs。除白细胞介素6含量外，Tsmu溶液保存样本的生物力学特性优于玻璃化保存，在体内低于新鲜OCAs。因此，Tsmu溶液可提供合适的保存条件，提高软骨细胞活力、保持OCA软骨基质结构完整并改善移植效果。
