The Seventh Department of Plastic Surgery, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 33, Badachu Road, Shijingshan District, Beijing, 100144, China.
Cell Tissue Bank. 2022 Dec;23(4):887-897. doi: 10.1007/s10561-022-10004-w. Epub 2022 Apr 6.
Osteochondral grafts are suitable materials for repair of articular cartilage defect and plastic and reconstructive surgery. In our study, osteochondral allografts from rabbits were preserved in vitro for 28 days, and chondrocyte death, degradation of collagen and proteoglycan, morphological alterations, and inflammatory reaction were observed in the grafts. Supplementing of Aucubin with 10 or 20 μM in the preservation solution inhibited chondrocyte death, matrix degradation, pathological alterations and inflammation in allografts preserved in vitro, compared with that preserved in standard preservation solution. In addition, after transplantation of 20 μM Aucubin-treated allografts, the osteochondral repair and regeneration of rabbits with knee joint defect were accelerated. In conclusion, Aucubin was beneficial for maintaining chondrocyte viability and normal morphology, and inhibiting inflammatory occurrence in rabbit osteochondral grafts preserved in vitro, and facilitated osteochondral repair and regeneration of rabbits with knee defect. These findings might provide novel insights for preservation of grafts for clinical articular cartilage repair and plastic surgery.
软骨移植是修复关节软骨缺损和整形重建的理想材料。本研究通过体外保存兔同种异体软骨 28 天，观察软骨移植物中的软骨细胞死亡、胶原和蛋白聚糖降解、形态改变和炎症反应。保存液中添加 10 或 20μM 桃叶珊瑚苷可抑制体外保存的同种异体软骨中软骨细胞死亡、基质降解、病理改变和炎症，与标准保存液相比。此外，在移植 20μM 桃叶珊瑚苷处理的同种异体移植物后，加速了兔膝关节缺损模型的软骨修复和再生。总之，桃叶珊瑚苷有利于维持软骨细胞活力和正常形态，抑制体外保存兔软骨移植物中炎症的发生，并促进兔膝关节缺损模型的软骨修复和再生。这些发现可能为临床关节软骨修复和整形手术提供新的见解。
