Berisha Bajram, Schams Dieter, Rodler Daniela, Sinowatz Fred, Pfaffl Michael W
Department of Animal Biotechnology, Faculty of Agriculture and Veterinary, University of Prishtina, Pristina, Kosovo.
Animal Physiology and Immunology Weihenstephan, Technical University of Munich, Munich, Germany.
Mol Reprod Dev. 2018 Jul;85(7):622-634. doi: 10.1002/mrd.22999. Epub 2018 Jul 24.
The aim of this study was to characterize certain prostaglandin family members in the bovine corpus luteum (CL) during the estrous cycle and pregnancy. The CL tissue was assigned to the stages 1-2, 3-4, 5-7, 8-12, 13-16 and >18 days (after regression) of the estrous cycle and 1-2, 3-4, 6-7, and >8 months of pregnancy. In these samples, we investigated prostaglandin F2alpha (PTGF), prostaglandin E2 (PTGE), their receptors (PTGFR, PTGER2, and PTGER4), cyclooxygenase 2 (COX-2), PTGF synthase (PTGFS), and PTGE synthase (PTGES). The expression of messenger RNA (mRNA) was measured by reverse transcription quantitative polymerase chain reaction, hormones by enzyme immunoassay, and localization by immunohistochemistry. The mRNA expression of COX-2, PTGFS, and PTGES in CL during the early-luteal phase was high followed by a continuous and significant downregulation afterward, as well as during all phases of pregnancy. The concentration of PTGF in CL tissue was high during the early-luteal phase, decreased significantly in the mid-luteal phase, and increased again afterward. In contrast, the concentration of PTGE increased significantly during the late-luteal phase followed by a decrease during regression. The PTGE level increased again during late pregnancy. Immunohistochemically, the large granulose-luteal cells show strong staining for COX-2 and PTGES during the early-luteal stage followed by lower activity afterward. During pregnancy, most of the luteal cells were only weakly positive or negative. In conclusion, our results indicate that the examined prostaglandin family members are involved in the local mechanisms that regulate luteal function, specifically during CL formation, function, and regression and during pregnancy in the cow.
本研究的目的是对发情周期和妊娠期牛黄体（CL）中的某些前列腺素家族成员进行特征描述。CL组织被分为发情周期的第1 - 2天、3 - 4天、5 - 7天、8 - 12天、13 - 16天以及退化后>18天，以及妊娠期的1 - 2个月、3 - 4个月、6 - 7个月和>8个月。在这些样本中，我们研究了前列腺素F2α（PTGF）、前列腺素E2（PTGE）、它们的受体（PTGFR、PTGER2和PTGER4）、环氧化酶2（COX - 2）、PTGF合酶（PTGFS）和PTGE合酶（PTGES）。通过逆转录定量聚合酶链反应测量信使核糖核酸（mRNA）的表达，通过酶免疫测定法检测激素，并通过免疫组织化学进行定位。COX - 2、PTGFS和PTGES在黄体早期的CL中的mRNA表达较高，随后持续且显著下调，在妊娠的所有阶段也是如此。CL组织中PTGF的浓度在黄体早期较高，在黄体中期显著下降，之后再次升高。相比之下，PTGE的浓度在黄体后期显著增加，在退化期下降。PTGE水平在妊娠后期再次升高。免疫组织化学显示，大颗粒黄体细胞在黄体早期对COX - 2和PTGES呈强染色，随后活性降低。在妊娠期间，大多数黄体细胞仅呈弱阳性或阴性。总之，我们的结果表明，所检测的前列腺素家族成员参与调节黄体功能的局部机制，特别是在奶牛CL的形成、功能、退化以及妊娠期间。
