Neuhaus Herbert, Gaul Florian, Hollemann Thomas
Martin-Luther-University Halle-Wittenberg, Institute for Physiological Chemistry, Halle (Saale), Germany.
Int J Dev Biol. 2018;62(4-5):319-324. doi: 10.1387/ijdb.170295hn.
LRPAP1, also known as receptor associated protein (RAP) is a small protein of 40 kDa associated with six of the seven members of the evolutionary conserved family of LDL receptors. Numerous studies showed that LRPAP1 has a dual function, initially as a chaperone insuring proper formation of intermolecular disulfide bonds during biogenesis of low density lipoprotein (LDL) receptors and later as an escort protein during trafficking through the endoplasmic reticulum and the early Golgi compartment, preventing premature interaction of receptor and ligand. Because of the general influence of LRPAP1 protein on lipid metabolism, we analyzed the temporal and spatial expression of the Xenopus laevis ortholog of lrpap1. Here, we show that lrpap1 was expressed in the developing neural system, the eye and ear anlagen, the branchial arches, the developing skin and the pronephric kidney. The very high expression level of lrpap1 specifically in the proximal tubules of the developing pronephros establishes this gene as a novel marker for the analysis of pronephros formation.
LRPAP1，也被称为受体相关蛋白（RAP），是一种40 kDa的小蛋白，与低密度脂蛋白受体进化保守家族的七个成员中的六个相关。大量研究表明，LRPAP1具有双重功能，最初作为伴侣蛋白，确保低密度脂蛋白（LDL）受体生物合成过程中分子间二硫键的正确形成，后来作为护送蛋白，在通过内质网和早期高尔基体区室的运输过程中，防止受体和配体过早相互作用。由于LRPAP1蛋白对脂质代谢的普遍影响，我们分析了非洲爪蟾lrpap1直系同源物的时空表达。在这里，我们表明lrpap1在发育中的神经系统、眼和耳原基、鳃弓、发育中的皮肤和前肾中表达。lrpap1在发育中的前肾近端小管中非常高的表达水平，使该基因成为分析前肾形成的一个新标记。
