解偶联蛋白3基因多态性及其与鸡饲料效率的关联

Polymorphisms in the uncoupling protein 3 gene and their associations with feed efficiency in chickens.

作者信息

Jin Sihua, Yang Lei, He Tingting, Fan Xinfeng, Wang Yiqiu, Ge Kai, Geng Zhaoyu

机构信息

College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.

出版信息

Asian-Australas J Anim Sci. 2018 Sep;31(9):1401-1406. doi: 10.5713/ajas.18.0217. Epub 2018 May 31.

DOI:10.5713/ajas.18.0217

PMID:29879809

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6127571/

Abstract

OBJECTIVE

The uncoupling protein 3 (UCP3) is a member of the mitochondrial anion carrier superfamily and has crucial effects on growth and feed efficiency in many species. Therefore, the objective of the present study was to examine the association of polymorphisms in the UCP3 gene with feed efficiency in meat-type chickens.

METHODS

Six single nucleotide polymorphisms (SNPs) of the UCP3 gene were chosen to be genotyped using matrix-assisted laser desorption-ionization time-of-flight mass spectrometry in meat-type chicken populations with 724 birds in total. Body weight at 49 (BW49) and 70 days of age (BW70) and feed intake (FI) in the interval were collected, then body weight gain (BWG) and feed conversion ratio (FCR) were calculated individually.

RESULTS

One SNP with a low minor allele frequency (<1%) was removed by quality control and data filtering. The results showed that rs13997809 of UCP3 was significantly associated with BWG and FCR (p<0.05), and that rs13997811 had significant effects on BW70 and BWG (p<0.05). Rs13997812 of UCP3 was strongly associated with BW70, FI, and FCR (p<0.05). Furthermore, individuals with AA genotype of rs13997809 had significantly higher BWG and lower FCR (p<0.05) than those with AT genotype. The GG individuals showed strongly higher BW70 and BWG than AA birds in rs13997811 (p<0.05). Birds with the TT genotype of rs13997812 had significantly greater BW70 and lower FCR compared with the CT birds (p<0.05). In addition, the TAC haplotype based on rs13997809, rs13997811, and rs13997812 showed significant effects on BW70, FI, and FCR (p<0.05).

CONCLUSION

Our results therefore demonstrate important roles for UCP3 polymorphisms in growth and feed efficiency that might be used in meat-type chicken breeding programs.

摘要

目的

解偶联蛋白3（UCP3）是线粒体阴离子载体超家族的成员，对许多物种的生长和饲料效率有重要影响。因此，本研究的目的是检测UCP3基因多态性与肉用型鸡饲料效率之间的关联。

方法

选择UCP3基因的6个单核苷酸多态性（SNP），采用基质辅助激光解吸电离飞行时间质谱法对总共724只肉用型鸡群体进行基因分型。收集49日龄（BW49）和70日龄（BW70）时的体重以及该时间段内的采食量（FI），然后分别计算体重增加量（BWG）和饲料转化率（FCR）。

结果

通过质量控制和数据过滤，去除了一个次要等位基因频率较低（<1%）的SNP。结果表明，UCP3的rs13997809与BWG和FCR显著相关（p<0.05），rs13997811对BW70和BWG有显著影响（p<0.05）。UCP3的rs13997812与BW70、FI和FCR密切相关（p<0.05）。此外，rs13997809的AA基因型个体的BWG显著高于AT基因型个体，FCR显著低于AT基因型个体（p<0.05）。在rs13997811中，GG基因型个体的BW70和BWG显著高于AA基因型个体（p<0.05）。rs13997812的TT基因型个体的BW70显著高于CT基因型个体，FCR显著低于CT基因型个体（p<0.05）。此外，基于rs13997809、rs13997811和rs13997812的TAC单倍型对BW70、FI和FCR有显著影响（p<0.05）。