Cas4 之谜的新见解

Insights into a Mysterious CRISPR Adaptation Factor, Cas4.

机构信息

Department of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109, USA.

出版信息

Mol Cell. 2018 Jun 7;70(5):757-758. doi: 10.1016/j.molcel.2018.05.028.

DOI:10.1016/j.molcel.2018.05.028

Abstract

Cas4 nucleases are conserved factors in many CRISPR systems, yet their molecular role has remained enigmatic. In this issue of Molecular Cell, Shiimori et al. (2018) report that two Cas4 nucleases together determine the size, orientation, and PAM for foreign DNA snippets acquired by CRISPR loci as immunological memory.

摘要

Cas4 核酸酶是许多 CRISPR 系统中保守的因素，但它们的分子作用一直是个谜。在本期《分子细胞》中，Shimori 等人（2018）报告称，两种 Cas4 核酸酶共同决定了 CRISPR 基因座获得的作为免疫记忆的外源 DNA 片段的大小、方向和 PAM。