Benalal D, Bachrach U
Biochem J. 1985 Apr 15;227(2):389-95. doi: 10.1042/bj2270389.
In cultured NG 108-15 neuroblastoma x glioma cells, opiates decreased cellular cyclic AMP and polyamine levels. This decrease was related to the inhibition of ornithine decarboxylase and cyclic AMP-dependent protein kinase activities during the acute exposure of the cells to the drugs. Growing the cells in the presence of opiates for several days led to drug addiction. In the tolerant-addicted cells, polyamine and cyclic AMP levels were close to normal values as were the activities of ornithine decarboxylase and cyclic AMP-dependent protein kinase. Removal of the opiate from 'addicted' cells, by either washing or by adding the antagonist naloxone, resulted in an increase in cyclic AMP and polyamine levels and the activities of ornithine decarboxylase and cyclic AMP-dependent protein kinase. The effect of opiates was closely related to their biological activities. Inactive enantiomorphs did not affect cyclic AMP or polyamine levels; neither did they decrease ornithine decarboxylase and cyclic AMP-dependent protein kinase activities.
在培养的NG 108 - 15神经母细胞瘤x胶质瘤细胞中,阿片类药物降低了细胞内环磷酸腺苷(cAMP)和多胺水平。这种降低与细胞急性暴露于药物期间鸟氨酸脱羧酶和环磷酸腺苷依赖性蛋白激酶活性的抑制有关。在阿片类药物存在的情况下培养细胞数天会导致药物成瘾。在耐受 - 成瘾的细胞中,多胺和环磷酸腺苷水平接近正常,鸟氨酸脱羧酶和环磷酸腺苷依赖性蛋白激酶的活性也是如此。通过洗涤或添加拮抗剂纳洛酮从“成瘾”细胞中去除阿片类药物,会导致环磷酸腺苷和多胺水平以及鸟氨酸脱羧酶和环磷酸腺苷依赖性蛋白激酶的活性增加。阿片类药物的作用与其生物学活性密切相关。无活性的对映体不影响环磷酸腺苷或多胺水平;它们也不会降低鸟氨酸脱羧酶和环磷酸腺苷依赖性蛋白激酶的活性。