Whitlon D S, Anderson K E, Mueller G C
Biochim Biophys Acta. 1985 Jul 9;835(2):369-77. doi: 10.1016/0005-2760(85)90293-0.
Phosphatidylcholine synthesis in cultured bovine lymphocytes is stimulated by cis-unsaturated fatty acids. This stimulation is correlated with an activation of the enzyme cytidyltransferase (EC 2.7.7.15) and its apparent translocation from the cytosol to the membrane/particulate of cells. In addition, these agents increase the levels of cytidine diphosphocholine - a product of the cytidyltransferase reaction and a precursor to phosphatidylcholine. Retinoic acid and 5,8,11,14-eicosatetraynoic acid both activate cytidyltransferase activity and raise cytidine diphosphocholine levels, yet they are ineffective as stimulators of overall phosphatidylcholine synthesis. The effects of all of these lipids are reversed by the delayed addition of bovine serum albumin. The data point to the view that cytidyltransferase activation is required but is not sufficient for stimulation of phosphatidylcholine synthesis: regulation at another step is suggested.
顺式不饱和脂肪酸可刺激培养的牛淋巴细胞中的磷脂酰胆碱合成。这种刺激与胞苷转移酶（EC 2.7.7.15）的激活及其从细胞溶质到细胞膜/微粒的明显易位相关。此外，这些物质会增加胞苷二磷酸胆碱的水平，胞苷二磷酸胆碱是胞苷转移酶反应的产物，也是磷脂酰胆碱的前体。视黄酸和5,8,11,14-二十碳四烯酸都能激活胞苷转移酶活性并提高胞苷二磷酸胆碱水平，但它们作为整体磷脂酰胆碱合成的刺激剂无效。延迟添加牛血清白蛋白可逆转所有这些脂质的作用。数据表明，胞苷转移酶的激活是刺激磷脂酰胆碱合成所必需的，但并不充分：提示在另一步骤存在调节。
