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[裸趾利什曼原虫动质体大环状DNA的限制性图谱]

[Restriction map of maxicircle kinetoplastic DNA of Leishmania gymnodactyli].

作者信息

Kolesnikov A A, Maslov D A, Saf'ianova V M, Stetsenko M M

出版信息

Nauchnye Doki Vyss Shkoly Biol Nauki. 1985(4):23-6.

PMID:2988652
Abstract

Kinetoplast DNA (kDNA) of Leishmania gymnodactyli, as in other Trypanosoma species, is a complicated associate consisting of mini- and maxicircular molecules. Minicircular DNA are presented by the homogeneous in size and heterogeneous in base sequence population. Their size determined by the agarose electrophoretic mobility in gel is 920 pairs of nucleotides. Maxicircular molecules of DNA are homogeneous and contains about 38 thou pairs of nucleotides. The restriction map of maxicircular kDNA molecule of L. gymnodactyli has been made up on the basis of the cleavage data of the whole associate kDNA by restrictases.

摘要

与其他锥虫物种一样,裸指利什曼原虫的动基体DNA(kDNA)是一种复杂的复合物,由微小环和大环分子组成。微小环DNA在大小上是均匀的,但碱基序列群体是异质的。通过琼脂糖凝胶电泳迁移率测定,其大小为920对核苷酸。大环DNA分子是均匀的,包含约38000对核苷酸。基于限制性内切酶对整个复合kDNA的切割数据,构建了裸指利什曼原虫大环kDNA分子的限制性图谱。

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