CIMAR/CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos s/n, 4450-208 Matosinhos, Portugal; Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
CIMAR/CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos s/n, 4450-208 Matosinhos, Portugal.
Mar Pollut Bull. 2018 Jun;131(Pt A):620-632. doi: 10.1016/j.marpolbul.2018.04.063. Epub 2018 May 4.
Hydrocarbon degradation (HD) potential by autochthonous microorganisms in the coastal sediments of the NW area of Portugal (coastal sandy beaches and estuaries of the rivers Minho and Douro) was evaluated, investigating if water and sediment contamination/characteristics influence it. Sediments were characterized for microbial abundance (by DAPI), HD microorganisms' abundance (by MPN), microbial community structure (by ARISA), hydrocarbons (by FTIR and SPME-GC-MS), hazardous and noxious substances (SPME-GC-MS) and metals (by AAS). To our knowledge, this is the first time all these pollutants, including the selected HNS, were measured simultaneously in sediments of the selected coastal area. Higher contaminants concentrations were, generally, registered in Douro samples. A clear differentiation of the microbial community structure between beaches and estuaries was observed, as well as, between Douro and Minho river estuaries. BIO-ENV analysis indicated both sediment characteristics (e.g. OM content) and contaminants presence/concentrations (e.g. tetrachloroethylene presence) affected the structure of the microbial community along the studied areas. In all the selected sites, the characterized autochthonous microbial communities showed potential for hydrocarbons degradation, with HD microorganisms being found in all collected sediments. These microorganisms can be a valuable asset to recover contaminated areas, but sediment characteristics and contaminants presence/levels need to be taken into account as they can affect their bioremediation potential and the success of their application as biotechnological tool.
评估了葡萄牙西北部沿海地区（沿海沙滩和米尼奥河与杜罗河河口）的土著微生物对碳氢化合物的降解（HD）潜力，研究了水和沉积物污染/特征是否会影响这一过程。通过 DAPI 测定微生物丰度、MPN 测定 HD 微生物丰度、ARISA 测定微生物群落结构、FTIR 和 SPME-GC-MS 测定碳氢化合物、SPME-GC-MS 测定有害物质和有毒物质以及 AAS 测定金属元素，对沉积物进行了特性描述。据我们所知，这是首次在选定的沿海地区沉积物中同时测量所有这些污染物，包括选定的 HNS。杜罗河样品中的污染物浓度通常更高。观察到海滩和河口之间以及杜罗河和米尼奥河河口之间的微生物群落结构明显分化。BIO-ENV 分析表明，沉积物特性（如 OM 含量）和污染物的存在/浓度（如四氯乙烯的存在）都影响了研究区域内微生物群落的结构。在所选择的所有地点，所描述的土著微生物群落都表现出对碳氢化合物降解的潜力，所有采集的沉积物中都发现了 HD 微生物。这些微生物可以成为修复受污染地区的宝贵资源，但需要考虑沉积物特性和污染物的存在/水平，因为它们会影响其生物修复潜力和作为生物技术工具应用的成功。
