Department of Microbiology, University of Lagos, Lagos, Nigeria.
O.N. Allen Laboratory for Soil Microbiology, Department of Soil Science, University of Wisconsin-Madison, Madison, WI, USA.
Appl Microbiol Biotechnol. 2017 May;101(10):4299-4314. doi: 10.1007/s00253-017-8151-6. Epub 2017 Feb 11.
Coastal sediments contaminated by polycyclic aromatic hydrocarbons (PAHs) can be candidates for remediation via an approach like land farming. Land farming converts naturally anaerobic sediments to aerobic environments, and the response of microbial communities, in terms of community structure alterations and corresponding effects on biodegradative activities, is unknown. A key goal of this study was to determine if different sediments exhibited common patterns in microbial community responses that might serve as indicators of PAH biodegradation. Sediments from three stations in the Lagos Lagoon (Nigeria) were used in microcosms, which were spiked with a mixture of four PAH, then examined for PAH biodegradation and for shifts in microbial community structure by analysis of diversity in PAH degradation genes and Illumina sequencing of 16S rRNA genes. PAH biodegradation was similar in all sediments, yet each exhibited unique microbiological responses and there were no microbial indicators of PAH bioremediation common to all sediments.
受多环芳烃 (PAHs) 污染的沿海沉积物可以通过土地耕作等方法进行修复。土地耕作将自然厌氧沉积物转化为需氧环境，微生物群落的响应，包括群落结构的改变和对生物降解活性的相应影响，尚不清楚。本研究的一个主要目标是确定不同的沉积物是否表现出微生物群落响应的常见模式，这些模式可能作为 PAH 生物降解的指标。该研究使用来自尼日利亚拉各斯泻湖 (Lagos Lagoon) 的三个站位的沉积物进行微宇宙实验，向其中添加四种 PAH 的混合物，然后通过分析 PAH 降解基因的多样性和 16S rRNA 基因的 Illumina 测序来检测 PAH 生物降解和微生物群落结构的变化。所有沉积物中的 PAH 生物降解情况相似，但每种沉积物都表现出独特的微生物响应，没有一种微生物可以作为所有沉积物中 PAH 生物修复的共同指标。
