Robert-Guroff M, Clark J W
Cancer Res. 1985 Jul;45(7):3374-7.
Monoclonal antibody HT462 recognizes a human T-cell leukemia virus type I (HTLV-I)-associated Mr 52,000 glycoprotein (HA-gp52), which is found on the surface of HTLV-infected cells. Whether HA-gp52 is encoded by the virus or by the infected cells has not yet been established. Using monoclonal HT462 in a competitive binding assay, natural human antibodies specific for HA-gp52 were detected in 97% of the patients from the United States, the Caribbean, and Japan with adult T-cell leukemia but not in healthy donors not exposed to HTLV-I. In contrast, antibodies to HA-gp52 occur in healthy virus-exposed donors, but at a lower prevalence than that observed in patients. Among Japanese from HTLV-I-endemic areas and exposed to the virus as indicated by the presence of antibodies to disrupted HTLV-I, 93% of adult T-cell leukemia patients were also seropositive for HA-gp52 compared to only 16% of healthy individuals. Differing sensitivities in the methods of assaying antibodies to HTLV and HA-gp52 were not responsible for these observations as shown by the lack of correlation of HTLV-I antibody titer with the presence of antibody to HA-gp52. Among adult T-cell leukemia patients, antibody titers to HTLV-I and HA-gp52 also varied independently. These results indicate that HA-gp52 in humans is antigenic and correlated with disease. Detection of antibody to this protein in asymptomatic individuals may be indicative of a predisease condition.
单克隆抗体HT462识别一种与人类I型T细胞白血病病毒（HTLV-I）相关的52000分子量糖蛋白（HA-gp52），该蛋白存在于HTLV感染细胞的表面。HA-gp52是由病毒编码还是由感染细胞编码尚未确定。在竞争性结合试验中使用单克隆抗体HT462，在美国、加勒比地区和日本的97%成人T细胞白血病患者中检测到了对HA-gp52具有特异性的天然人类抗体，而在未接触HTLV-I的健康供者中未检测到。相比之下，在接触过病毒的健康供者中也存在针对HA-gp52的抗体，但其患病率低于患者。在来自HTLV-I流行地区且如针对灭活HTLV-I的抗体所示接触过该病毒的日本人中，93%的成人T细胞白血病患者HA-gp52血清学检测也呈阳性，而健康个体中只有16%呈阳性。如HTLV-I抗体滴度与HA-gp52抗体的存在缺乏相关性所示，检测HTLV和HA-gp52抗体的方法存在不同敏感性并非导致这些观察结果的原因。在成人T细胞白血病患者中，针对HTLV-I和HA-gp52的抗体滴度也独立变化。这些结果表明，人类中的HA-gp52具有抗原性且与疾病相关。在无症状个体中检测该蛋白的抗体可能预示着疾病前期状态。
