Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing, 100093, China.
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
Mol Nutr Food Res. 2018 Sep;62(17):e1800080. doi: 10.1002/mnfr.201800080. Epub 2018 Aug 5.
Dietary supplementation with polyphenol-rich propolis can protect against experimentally induced colitis. We examined whether different polyphenol compositions of Chinese propolis (CP) and Brazilian propolis (BP) influence their ability to protect against dextran sulfate sodium (DSS)-induced colitis in rats.
HPLC-DAD/Q-TOF-MS analysis confirmed that polyphenol compositions of CP and BP were dissimilar. Rats were given CP or BP by gavage (300 mg kg body weight) throughout the study, starting 1 week prior to DSS treatment for 1 week followed by 3 d without DSS. CP and BP significantly reduced the colitis disease activity index relative to controls not receiving propolis, prevented significant DSS-induced colonic tissue damage, and increased resistance to DSS-induced colonic oxidative stress as shown by reduced malonaldehyde levels and increased T-AOC levels. CP and BP significantly reduced DSS-induced colonic apoptosis. Colonic inflammatory markers IL-1β, IL-6, and MCP-1 were suppressed by CP and BP, whereas only BP-induced expression of TGF-β. CP, not BP, increased the diversity and richness of gut microbiota populations. Both forms of propolis significantly reduced populations of Bacteroides spp.
Despite the dissimilar polyphenol compositions of CP and BP, their ability to protect against DSS-induced colitis is similar. Nevertheless, some different physiological impacts were observed.
富含多酚的蜂胶的饮食补充可以预防实验性诱导的结肠炎。我们研究了中国蜂胶 (CP) 和巴西蜂胶 (BP) 的不同多酚组成是否会影响它们预防葡聚糖硫酸钠 (DSS) 诱导的大鼠结肠炎的能力。
HPLC-DAD/Q-TOF-MS 分析证实 CP 和 BP 的多酚组成不同。在 DSS 处理前 1 周开始，通过灌胃给予大鼠 CP 或 BP（300mg/kg 体重），持续整个研究期间，共 1 周，随后连续 3 天不给予 DSS。CP 和 BP 与未接受蜂胶的对照组相比，显著降低了结肠炎疾病活动指数，预防了 DSS 诱导的结肠组织损伤，并且增加了对 DSS 诱导的结肠氧化应激的抵抗力，表现为降低了丙二醛水平和增加了 T-AOC 水平。CP 和 BP 显著降低了 DSS 诱导的结肠细胞凋亡。CP 和 BP 抑制了结肠炎性标志物 IL-1β、IL-6 和 MCP-1 的表达，而仅 BP 诱导了 TGF-β的表达。CP 而不是 BP 增加了肠道微生物群落的多样性和丰富度。两种形式的蜂胶都显著降低了拟杆菌属的种群。
尽管 CP 和 BP 的多酚组成不同，但它们预防 DSS 诱导的结肠炎的能力相似。然而，观察到了一些不同的生理影响。
