Perera Wilmer H, Ramsaroop Taylor, Carvalho Raquel, Rodenburg Douglas L, McChesney James D
a Ironstone Separations, Inc. , Oxford , MS , USA.
b Universidade Federal de Mato Grosso do Sul , Campo Grande , Brazil.
Nat Prod Res. 2019 Jul;33(13):1876-1884. doi: 10.1080/14786419.2018.1478826. Epub 2018 Jun 11.
A silica gel orthogonal method using acetonitrile: water was developed for the analyses of fractions rich in very polar steviol glycosides and resolve regions of co-elution of these compounds in reversed-phase. Additionally, we also used this normal phase analytical method to scale up the purification process of steviol glycosides. Using these approaches, one novel minor tetra-glucopyranosyl diterpene glycosides together with three known compounds were purified from a commercial leaf extract. Compound was unambiguously elucidated as 13-[(2--β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]-kaur-16-en-19-oic acid-(6--β-D-glucopyranosyl-β-D-glucopyranosyl) ester (rebaudioside Y) based on high-performance liquid chromatography retention times, tandem mass spectrometry dissociation pattern and 1D and 2D NMR experiments. Known compounds were isolated in gram quantities and identified as rebaudioside D, E and M.
水的硅胶正交方法，用于分析富含高极性甜菊糖苷的馏分，并解决这些化合物在反相色谱中的共洗脱区域问题。此外，我们还使用这种正相分析方法来扩大甜菊糖苷的纯化过程。使用这些方法，从商业叶提取物中纯化出一种新型的次要四葡萄糖基二萜糖苷以及三种已知化合物。基于高效液相色谱保留时间、串联质谱解离模式以及一维和二维核磁共振实验，化合物被明确鉴定为13-[(2--β-D-吡喃葡萄糖基-β-D-吡喃葡萄糖基)氧基]-贝壳杉-16-烯-19-酸-(6--β-D-吡喃葡萄糖基-β-D-吡喃葡萄糖基)酯（莱鲍迪苷Y）。已知化合物以克级量分离出来，并鉴定为莱鲍迪苷D、E和M。
