Dadssi M, Cozzone A J
FEBS Lett. 1985 Jul 8;186(2):187-90. doi: 10.1016/0014-5793(85)80705-5.
The effect of cyclic AMP on protein phosphorylation was analyzed comparatively in two strains of E.coli differing in their capacity to synthesize this nucleotide, one of them lacking the adenylate cyclase activity. The results obtained from both in vivo and in vitro experiments concurred in showing that the bacterial protein kinase activity is cAMP-independent.
在两种合成这种核苷酸能力不同的大肠杆菌菌株中,对环磷酸腺苷(cAMP)对蛋白质磷酸化的影响进行了比较分析,其中一种菌株缺乏腺苷酸环化酶活性。体内和体外实验获得的结果一致表明,细菌蛋白激酶活性不依赖于cAMP。