Zhang Yun, Wang Yan, Sosic Zoran, Zang Li, Bergelson Svetlana, Zhang Wei
Analytical Development, Biogen Inc, Cambridge, MA, USA.
Analytical Development, Biogen Inc, Cambridge, MA, USA.
Anal Biochem. 2018 Aug 15;555:22-25. doi: 10.1016/j.ab.2018.06.006. Epub 2018 Jun 8.
A simple and rapid identity test of adeno-associated virus (AAV) serotypes is important for supporting the AAV gene therapy development, as it relates to its efficacy and safety. The current mass spectrometry-based identity tests require extensive sample preparation steps, relatively large sample quantities and long analysis time. Herein, we describe a simple and novel microfluidic ZipChip CE/MS method used to characterize AAV capsid proteins. The three capsid proteins of AAV2 were separated and identified within 4 min using 5 nL of sample directly from a polysorbate-containing formulation buffer. This rapid method can be suitable to confirm AAV serotype identity.
腺相关病毒（AAV）血清型的简单快速鉴定测试对于支持AAV基因治疗的发展很重要，因为这关系到其疗效和安全性。当前基于质谱的鉴定测试需要大量的样品制备步骤、相对大量的样品以及较长的分析时间。在此，我们描述了一种简单新颖的微流控ZipChip CE/MS方法，用于表征AAV衣壳蛋白。使用直接来自含聚山梨酯的制剂缓冲液的5 nL样品，在4分钟内分离并鉴定了AAV2的三种衣壳蛋白。这种快速方法可适用于确认AAV血清型。
