Department of Environmental Health, School of Public Health, Nantong University, Nantong, 226001, China.
The Third People's Hospital of Nantong, Nantong, 226006, China.
Biochem Biophys Res Commun. 2018 Oct 2;504(2):393-399. doi: 10.1016/j.bbrc.2018.06.029. Epub 2018 Jun 19.
Arsenic is associated with several adverse health outcomes, and people with diabetes may be more susceptible to arsenic. In this study, we found that arsenic levels in some tissues such as liver, kidney, and heart but not lung of type 1 diabetes mellitus (T1DM) mice were higher than in those of normal mice after a single oral dose of arsenic trioxide for 2 h. However, little is known about the molecular mechanism of the increased tissue uptake of trivalent inorganic arsenic in mice with T1DM. This study aimed to investigate the expression of the mammalian arsenic transporters aquaglyceroporins (AQPs) and glucose transporter 1 (GLUT1) in T1DM mice and compare them with those in normal mice. Results showed that the levels of AQP9 and GLUT1 mRNA and protein were higher in T1DM mouse liver than in the normal one. The levels of AQP7 mRNA and protein were higher in T1DM mouse kidney. In the heart, we observed that the levels of AQP7 and GLUT1 mRNA and protein were higher in T1DM mice, but the levels of AQP9 mRNA and protein in the lung had no significant difference between both mice. These results suggested that T1DM may increase the expression of transporters of trivalent inorganic arsenic and thus increase the arsenic uptake in specific tissues.
砷与多种健康不良后果有关，而糖尿病患者可能更容易受到砷的影响。在这项研究中，我们发现，在给予三氧化二砷单次口服 2 小时后，1 型糖尿病（T1DM）小鼠的一些组织（如肝脏、肾脏和心脏）中的砷水平高于正常小鼠，但肺组织中的砷水平没有差异。然而，对于 T1DM 小鼠中三价无机砷在组织中摄取增加的分子机制知之甚少。本研究旨在探讨 T1DM 小鼠中哺乳动物砷转运蛋白水通道蛋白 9（AQP9）和葡萄糖转运蛋白 1（GLUT1）的表达情况，并与正常小鼠进行比较。结果表明，T1DM 小鼠肝脏中 AQP9 和 GLUT1mRNA 和蛋白的水平高于正常小鼠。T1DM 小鼠肾脏中 AQP7mRNA 和蛋白的水平较高。在心脏中，我们观察到 T1DM 小鼠中 AQP7 和 GLUT1mRNA 和蛋白的水平较高，但肺组织中 AQP9mRNA 和蛋白的水平在两种小鼠之间没有显著差异。这些结果表明，T1DM 可能会增加三价无机砷转运蛋白的表达，从而增加特定组织对砷的摄取。
