Weyer B, Sonne O
Mol Cell Endocrinol. 1985 Jun;41(1):85-92. doi: 10.1016/0303-7207(85)90145-5.
In cultured human lymphocytes (IM-9) and in isolated rat adipocytes human growth hormone is substrate for a receptor-mediated degradation. When the cells are incubated with monoiodinated human growth hormone half of the radioactivity dissociating from the cells is in the form of [125I]monoiodotyrosine. Since IM-9 lymphocytes have no receptor-mediated degradation of insulin, obviously insulin and human growth hormone follow different pathways in this cell type. In the rat adipocyte colchicine and monodansylcadaverine caused quantitatively different uptake and degradation of these 2 ligands suggesting that also in this cell type the pathways are functionally different. The application of different inhibitors suggests that the receptor-mediated degradation of growth hormone in these 2 cell types takes place in an acidified compartment by an energy-requiring process and involving thiol groups.
在培养的人淋巴细胞（IM - 9）和分离的大鼠脂肪细胞中，人生长激素是受体介导降解的底物。当细胞与单碘化人生长激素一起孵育时，从细胞中解离的放射性物质的一半是以[125I]单碘酪氨酸的形式存在。由于IM - 9淋巴细胞对胰岛素没有受体介导的降解作用，显然在这种细胞类型中胰岛素和人生长激素遵循不同的途径。在大鼠脂肪细胞中，秋水仙碱和单丹磺酰尸胺对这两种配体的摄取和降解在数量上有所不同，这表明在这种细胞类型中途径在功能上也是不同的。不同抑制剂的应用表明，这两种细胞类型中生长激素的受体介导降解发生在酸化区室中，是一个需要能量的过程，并且涉及巯基。
