从放线菌根瘤固氮细菌弗兰克氏菌属中克隆多拷贝质粒及其限制性图谱的测定。

Cloning of a multicopy plasmid from the actinorhizad nitrogen-fixing bacterium Frankia sp. and determination of its restriction map.

作者信息

Normand P, Downie J A, Johnston A W, Kieser T, Lalonde M

出版信息

Gene. 1985;34(2-3):367-70. doi: 10.1016/0378-1119(85)90147-7.

Abstract

An 8.3-kb multicopy plasmid, pFQ31, from the nitrogen-fixing Frankia sp. strain ArI3, was cloned into Escherichia coli plasmid vectors and analysed physically. pFQ31 has no detectable sequence homology with another Frankia plasmid, pFQ32, which is present in the same host. Derivatives of pFQ31 with an antibotic resistance marker were introduced into Streptomyces lividans, which is taxonomically related to Frankia, but no stable replication could be achieved.

摘要

来自固氮放线菌Frankia sp.菌株ArI3的一个8.3千碱基对的多拷贝质粒pFQ31，被克隆到大肠杆菌质粒载体中并进行了物理分析。pFQ31与存在于同一宿主中的另一个Frankia质粒pFQ32没有可检测到的序列同源性。带有抗生素抗性标记的pFQ31衍生物被导入与Frankia有分类学关系的变铅青链霉菌中，但未能实现稳定复制。