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用低成本电路检测细胞培养分析物。

Sensing Cell-Culture Assays with Low-Cost Circuitry.

机构信息

Instituto de Microelectrónica de Sevilla, IMSE, Universidad de Sevilla, Av. Américo Vespucio sn, 41092, Sevilla, Spain.

Dto. Tecnología Electrónica, Escuela Técnica Superior de Ingeniería Informática, Universidad de Sevilla, Av. Reina Mercedes sn, 41012, Sevilla, Spain.

出版信息

Sci Rep. 2018 Jun 11;8(1):8841. doi: 10.1038/s41598-018-27295-3.

DOI:10.1038/s41598-018-27295-3
PMID:29892009
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5995807/
Abstract

An alternative approach for cell-culture end-point protocols is proposed herein. This new technique is suitable for real-time remote sensing. It is based on Electrical Cell-substrate Impedance Spectroscopy (ECIS) and employs the Oscillation-Based Test (OBT) method. Simple and straightforward circuit blocks form the basis of the proposed measurement system. Oscillation parameters - frequency and amplitude - constitute the outcome, directly correlated with the culture status. A user can remotely track the evolution of cell cultures in real time over the complete experiment through a web tool continuously displaying the acquired data. Experiments carried out with commercial electrodes and a well-established cell line (AA8) are described, obtaining the cell number in real time from growth assays. The electrodes have been electrically characterized along the design flow in order to predict the system performance and the sensitivity curves. Curves for 1-week cell growth are reported. The obtained experimental results validate the proposed OBT for cell-culture characterization. Furthermore, the proposed electrode model provides a good approximation for the cell number and the time evolution of the studied cultures.

摘要

本文提出了一种用于细胞培养终点协议的替代方法。这项新技术适用于实时远程感应。它基于电细胞-基底阻抗谱（ECIS）并采用基于振荡的测试（OBT）方法。简单而直接的电路模块构成了所提出的测量系统的基础。振荡参数 - 频率和幅度 - 构成了结果，与培养状态直接相关。用户可以通过一个网络工具实时跟踪整个实验中细胞培养的演变，该工具持续显示所获取的数据。通过使用商业电极和成熟的细胞系（AA8）进行实验，从生长测定中实时获得细胞数量。已经沿着设计流程对电极进行了电特性表征，以预测系统性能和灵敏度曲线。报告了为期 1 周的细胞生长曲线。所获得的实验结果验证了所提出的 OBT 用于细胞培养特性的研究。此外，所提出的电极模型为研究培养物中的细胞数量和时间演变提供了很好的近似。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d1d/5995807/8fb7fd18e150/41598_2018_27295_Fig13_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d1d/5995807/e3a0af5179d8/41598_2018_27295_Fig5_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d1d/5995807/c7df88b90535/41598_2018_27295_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d1d/5995807/6cf4a2976bce/41598_2018_27295_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d1d/5995807/d9821ba93c8c/41598_2018_27295_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d1d/5995807/539fdd9f405c/41598_2018_27295_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d1d/5995807/065e8e9326d6/41598_2018_27295_Fig12_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d1d/5995807/8fb7fd18e150/41598_2018_27295_Fig13_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d1d/5995807/2ba2962e119b/41598_2018_27295_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d1d/5995807/5a62d3cd7561/41598_2018_27295_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d1d/5995807/db101a54f7e7/41598_2018_27295_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d1d/5995807/0e9b0ac11ed4/41598_2018_27295_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d1d/5995807/e3a0af5179d8/41598_2018_27295_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d1d/5995807/875bb6b6f91a/41598_2018_27295_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d1d/5995807/7befea7a2d29/41598_2018_27295_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d1d/5995807/c7df88b90535/41598_2018_27295_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d1d/5995807/6cf4a2976bce/41598_2018_27295_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d1d/5995807/d9821ba93c8c/41598_2018_27295_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d1d/5995807/539fdd9f405c/41598_2018_27295_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d1d/5995807/065e8e9326d6/41598_2018_27295_Fig12_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d1d/5995807/8fb7fd18e150/41598_2018_27295_Fig13_HTML.jpg

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