Jimenez Carolina Rodriguez, de Azevedo Jovana Luiza, Ciro Alexandre Alves Torres, Penitente-Filho Jurandy Mauro, Gonçalves Wagner Gonzaga
Department of Animal Science, Laboratory of Physiology and Animal Reproduction, Federal University of Viçosa, Viçosa, Brazil.
Department of Biology, Laboratory of Cellular Ultrastructure, Federal University of Viçosa, Viçosa, Brazil.
Reprod Domest Anim. 2018 Oct;53(5):1103-1113. doi: 10.1111/rda.13210. Epub 2018 Jun 12.
The growth hormone (GH) and growth insulin-like factor-1 (IGF-1) act directly upon the regulation and growth in the different phases of preantral follicles. Thus, it is necessary to define their sequentiality until the in vitro preovulatory development. Therefore, the study aimed to assess the effects of a sequential medium containing GH and/or IGF-1 in the long-duration in vitro culture of preantral ovarian follicles. Ovarian fragments were cultivated: first half (days 1-7), second half (days 7-14) or during 14 culture days. Treatments were identified as: αMEM+; GH → IGF-1; IGF-1 → GH and GH + IGF-1. The culture was designed in 24-well plates, in an incubator at 37°C and 5% CO . The parameters of normality, viability, follicles (primordial/in developing) and follicle diameter were evaluated. In addition, the ultrastructure was confirmed with electron transmission microscopy. The results showed that the culture treated with GH → IGF-1 kept the follicular normality and the viability until the 14th day of culture and increased both in the follicular development until 7th day and in the follicular diameter until 14th day, when compared to the control. The treatments IGF-1 → GH and GH + IGF-1 were not effective in the developing and follicular diameter after 7 days of culture, and also reduced the percentage of viability. It is concluded that the bovine preantral follicles cultured in the sequential medium treated with GH → IGF-1 improved the follicular development until the first half of the culture and kept these parameters with normality, viability and ultrastructure until the second half of the in vitro culture.
生长激素（GH）和胰岛素样生长因子-1（IGF-1）直接作用于窦前卵泡不同阶段的调节和生长。因此，有必要确定它们在体外排卵前发育之前的先后顺序。因此，本研究旨在评估含有GH和/或IGF-1的序贯培养基在窦前卵巢卵泡长期体外培养中的作用。培养卵巢碎片：前半段（第1 - 7天）、后半段（第7 - 14天）或培养14天。处理方式分为：αMEM+；GH→IGF-1；IGF-1→GH和GH + IGF-1。培养在24孔板中进行，置于37°C、5% CO₂的培养箱中。评估正常性、活力、卵泡（原始/发育中）和卵泡直径等参数。此外，通过电子透射显微镜确认超微结构。结果表明，与对照组相比，用GH→IGF-1处理的培养物在培养第14天时保持卵泡正常性和活力，并且在卵泡发育至第7天时以及卵泡直径至第14天时均有所增加。IGF-1→GH和GH + IGF-1处理在培养7天后对卵泡发育和卵泡直径无效，并且还降低了活力百分比。结论是，在经GH→IGF-1处理的序贯培养基中培养的牛窦前卵泡在培养的前半段改善了卵泡发育，并在体外培养的后半段保持了这些参数的正常性、活力和超微结构。
