Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
J Eukaryot Microbiol. 2019 Mar;66(2):212-220. doi: 10.1111/jeu.12643. Epub 2018 Jul 1.
Resting cyst formation is a remarkable survival strategy used by ciliates in response to the adverse environmental conditions. However, the mechanisms underlying encystment are poorly understood. Here, the genetic basis of encystment in Colpoda aspera was examined through RNA sequencing to identify transcriptome-wide changes in gene expression between vegetative and encystment stages. After de novo assembly, 49,543 transcripts were identified. Gene annotation and pathway mapping analysis revealed marked changes in biosynthesis, energy metabolism, and autophagy pathways during cyst formation. In addition, some differentially regulated genes were predicted to function in the interconnected cAMP, AMPK, mTOR, and PI3K/AKT signaling pathways, potentially forming a regulatory network for encystment. The present study conducted a large-scale assessment of Colpoda aspera genomic resources and provides new insight into the molecular mechanisms underlying cyst formation.
休眠囊泡形成是纤毛虫应对不利环境条件的一种显著生存策略。然而，囊泡形成的机制还知之甚少。本研究通过 RNA 测序检查了粗糙尾柱虫的囊泡形成的遗传基础，以鉴定在营养体和囊泡形成阶段之间基因表达的全转录组变化。通过从头组装，鉴定出 49543 个转录本。基因注释和途径映射分析表明，在囊泡形成过程中生物合成、能量代谢和自噬途径发生了明显变化。此外，一些差异调控基因预测在相互连接的 cAMP、AMPK、mTOR 和 PI3K/AKT 信号通路中发挥作用，可能形成囊泡形成的调控网络。本研究对粗糙尾柱虫的基因组资源进行了大规模评估，为囊泡形成的分子机制提供了新的见解。
