Institut für Chemie und Biochemie, Freie Universität Berlin, Takustraße 3, 14195, Berlin, Germany.
Institute of Microbiology, Technische Universität Dresden, Zellescher Weg 20b, 01217, Dresden, Germany.
Chemistry. 2018 Aug 1;24(43):10966-10970. doi: 10.1002/chem.201802458. Epub 2018 Jul 3.
The design and construction of polymeric compartmentalized structures in water have been intensively explored for controllable catalysis, but there is still the challenge of setting up catalytic compartments in organic media. Here, we designed a simple block copolymer, PCL-b-PEG-b-PCL, to construct a stable and multi-compartmentalized emulsion in an organic solvent by hand-shaking. This gentle emulsion preparation allowed a successful encapsulation of vulnerable biocatalysts such as benzaldehyde lyase (BAL) and alcohol dehydrogenase (ADH). The compartmentalization provided the emulsion with an exceptionally large interfacial area that could enhance BAL activity up to 225 times as compared to the traditional biphasic system. Moreover, the system could be easily scaled up due to its facile preparation with low cost. Therefore, our results pave the way for developing compartmentalized structures in solvents for biocatalysis in large-scale synthetic chemistry.
在水中构建聚合隔室结构以用于可控催化的研究已经非常深入，但是在有机介质中设置催化隔室仍然具有挑战性。在这里，我们设计了一种简单的嵌段共聚物 PCL-b-PEG-b-PCL，通过手动摇晃在有机溶剂中构建了一种稳定的多隔室乳液。这种温和的乳液制备方法成功地封装了脆弱的生物催化剂，如苯甲醛裂解酶（BAL）和醇脱氢酶（ADH）。隔室化使乳液具有非常大的界面面积，与传统的两相系统相比，BAL 活性可提高 225 倍。此外，由于其制备简单且成本低，因此该系统可以很容易地放大。因此，我们的研究结果为在溶剂中开发用于大规模合成化学的生物催化的隔室结构铺平了道路。
