Xiong Y, Xiong Y Y, Xu Z G
Eur J Gynaecol Oncol. 2016 Aug;37(4):517-521.
The aim of this study was to explore a panel of useful markers in differential diagnosis of primary endocervical adenocarcinoma (ECA) and endometrial adenocarcinoma (EMA). Materials anid Methods: Thirty-three ECAs and 31 EMAs were collected and examined for high-risk human papillomavirus (hr-HPV) (16/18) DNA using in situ hybridization, and for p16, vimentin, ER, PR expression using immunohistochemistry (IHC).
Detection rate of hr-HPV (16/18) DNA in ECA (72.7%, 24/33) was significantly higher than that in EMA (12.9%, 4/31) (p < 0.01). Twenty-four of 33 (72.7%) cases of ECA, but only five of 31 (16.1%) cases of EMA showed high expression of pl6. Twenty-three of 24 (95.8%) hr-HPV DNA-positive ECA and all four (100.0%) hr-HPV DNA-positive EMA showed high levels of pl6 expression. High expression rates of vimentin (90.3%, 28/31), ER (58.1%, 18/31), and PR (71.0%, 22/31) in EMA were significantly higher than those in ECA, respectively (p < 0.01).
Detection of hr-HPV DNA combined with immunohistochemical expressions of pl6, vimentin, ER, and PR have important value in differential diagnosis between ECA and EMA.
本研究旨在探索一组有助于鉴别原发性宫颈腺癌（ECA）和子宫内膜腺癌（EMA）的有用标志物。材料与方法：收集33例ECA和31例EMA，采用原位杂交检测高危型人乳头瘤病毒（hr-HPV）（16/18）DNA，采用免疫组织化学（IHC）检测p16、波形蛋白、雌激素受体（ER）、孕激素受体（PR）的表达。
ECA中hr-HPV（16/18）DNA的检出率（72.7%，24/33）显著高于EMA（12.9%，4/31）（p<0.01）。33例ECA中有24例（72.7%）p16高表达，而31例EMA中只有5例（16.1%）p16高表达。24例hr-HPV DNA阳性的ECA中有23例（95.8%）p16表达水平高，4例（100.0%）hr-HPV DNA阳性的EMA均p16高表达。EMA中波形蛋白（90.3%，28/31）、ER（58.1%，18/31）和PR（71.0%，22/31）的高表达率分别显著高于ECA（p<0.01）。
检测hr-HPV DNA并结合p16、波形蛋白、ER和PR的免疫组化表达对ECA和EMA的鉴别诊断具有重要价值。
