Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao 266003, China.
Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao 266003, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China.
Fish Shellfish Immunol. 2018 Sep;80:443-451. doi: 10.1016/j.fsi.2018.06.018. Epub 2018 Jun 9.
The 94-kDa glucose-regulated protein (GRP94) belonging to the HSP90 family is an endoplasmic reticulum (ER) chaperone. It plays critical roles in ER quality control, and has been implicated as a specialized immune chaperone to regulate both innate and adaptive immunity. In this study, we identified and characterized a GRP94 gene (PyGRP94) from Yesso scallop (Patinopecten yessoensis). The protein sequence of PyGRP94 is highly conserved with its homologs in vertebrates, with a signal sequence in N-terminal, an ER retrieval signal sequence in C-terminal and a HATPase_c domain. Expression analysis suggests that PyGRP94 transcripts in early embryos are maternally derived and the zygotic expression is started from D-shaped larvae. This gene is also expressed in almost all the adult tissues examined except smooth muscle, with the highest expression level in hemocytes. Besides, PyGRP94 was demonstrated to be induced by heat shock and both Gram-positive (Micrococcus luteus) and Gram-negative (Vibrio anguillarum) bacterial infection, with much more dramatic changes being observed after V. anguillarum challenge. Our results suggest the involvement of PyGRP94 in response to thermal stress, and that it might play an important role in the innate immune defense of scallop.
属于 HSP90 家族的 94kDa 葡萄糖调节蛋白（GRP94）是内质网（ER）伴侣蛋白。它在 ER 质量控制中发挥着关键作用，并被认为是一种专门的免疫伴侣蛋白，可调节先天免疫和适应性免疫。在这项研究中，我们从虾夷扇贝（Patinopecten yessoensis）中鉴定和表征了 GRP94 基因（PyGRP94）。PyGRP94 的蛋白序列与脊椎动物的同源物高度保守，具有 N 端的信号序列、C 端的 ER 回收信号序列和 HATPase_c 结构域。表达分析表明，早期胚胎中的 PyGRP94 转录本是母源性的，合子表达从 D 形幼虫开始。该基因也在除平滑肌外的几乎所有成年组织中表达，在血细胞中的表达水平最高。此外，PyGRP94 被证明可被热休克和革兰氏阳性（微球菌）和革兰氏阴性（鳗弧菌）细菌感染诱导，在鳗弧菌攻击后观察到更明显的变化。我们的结果表明 PyGRP94 参与了对热应激的反应，并且它可能在扇贝的先天免疫防御中发挥重要作用。
